The enzymatic hydrolysis of O-glycosidic linkages is one of probably the most numerous and widespread reactions in nature and includes a traditional “textbook” enzyme mechanism. A multidisciplinary analysis of a beta-glycoside hydrolase, the Cel5A from Bacillus agaradhaerens, is offered during which the constructions of every of the native, substrate, covalent-intermediate, and product complexes have been… Continue reading
