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Glycosides and Glycosilation of Recombiant Proteins

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Gene-centric metagenomics of the fiber-adherent bovine rumen microbiome reveals forage specific glycoside hydrolases.

Gene-centric metagenomics of the fiber-adherent bovine rumen microbiome reveals forage specific glycoside hydrolases.

Posted on September 25, 2021July 25, 2021 by Mathew

The complicated microbiome of the rumen capabilities as an efficient system for the conversion of plant cell wall biomass to microbial protein, quick chain fatty acids, and gases. As such, it supplies a singular genetic useful resource for plant cell wall degrading microbial enzymes that could possibly be utilized in the manufacturing of biofuels. The rumen and gastrointestinal tract harbor a dense and complicated microbiome.

To achieve a larger understanding of the ecology and metabolic potential of this microbiome, we used comparative metagenomics (phylotype evaluation and SEED subsystems-based annotations) to look at randomly sampled pyrosequence knowledge from 3 fiber-adherent microbiomes and 1 pooled liquid pattern (a mix of the liquid microbiome fractions from the similar bovine rumens). Even although the Three animals have been fed the similar food plan, the group construction, predicted phylotype, and metabolic potentials in the rumen have been markedly totally different with respect to nutrient utilization.

A comparability of the glycoside hydrolase and cellulosome practical genes revealed that in the rumen microbiome, preliminary colonization of fiber seems to be by organisms possessing enzymes that assault the simply obtainable facet chains of complicated plant polysaccharides and never the extra recalcitrant most important chains, particularly cellulose.

Furthermore, in comparison with the termite hindgut microbiome, there are basic variations in the glycoside hydrolase content material that look like food plan pushed for both the bovine rumen (forages and legumes) or the termite hindgut (wooden).

Digoxin and different cardiac glycosides inhibit HIF-1alpha synthesis and block tumor development.

A library of medicine which can be in scientific trials or use was screened for inhibitors of hypoxia-inducible issue 1 (HIF-1). Twenty medicine inhibited HIF-1-dependent gene transcription by >88% at a focus of 0.Four microM. Eleven of these medicine have been cardiac glycosides, together with digoxin, ouabain, and proscillaridin A, which inhibited HIF-1alpha protein synthesis and expression of HIF-1 goal genes in most cancers cells.

Digoxin administration elevated latency and decreased development of tumor xenografts, whereas remedy of established tumors resulted in development arrest inside one week. Enforced expression of HIF-1alpha by transfection was not inhibited by digoxin, and xenografts derived from these cells have been immune to the anti-tumor results of digoxin, demonstrating that HIF-1 is a important goal of digoxin for most cancers remedy.

Gene-centric metagenomics of the fiber-adherent bovine rumen microbiome reveals forage specific glycoside hydrolases.

Cardiac glycosides are a various household of naturally derived compounds that bind to and inhibit Na+/Ok+-ATPase. Members of this household have been in scientific use for a few years for the remedy of coronary heart failure and atrial arrhythmia, and the mechanism of their constructive inotropic impact is nicely characterised. Exciting latest findings have prompt further signalling modes of motion of Na+/Ok+-ATPase, implicating cardiac glycosides in the regulation of a number of vital mobile processes and highlighting potential new therapeutic roles for these compounds in numerous ailments.

Perhaps most notably, the elevated susceptibility of most cancers cells to those compounds helps their potential use as most cancers therapies, and the first technology of glycoside-based anticancer medicine are presently in scientific trials.

Superior safety in opposition to malaria and melanoma metastases by a C-glycoside analogue of the pure killer T cell ligand alpha-Galactosylceramide.

alpha-Galactosylceramide (alpha-GalCer) is a glycolipid that stimulates pure killer T cells to supply each T helper (Th) 1 and Th2 cytokines. This property permits alpha-GalCer to ameliorate all kinds of infectious, neoplastic, and autoimmune ailments; nonetheless, its effectiveness in opposition to anybody illness is restricted by the opposing actions of the induced Th1 and Th2 cytokines. Here, we report {that a} artificial C-glycoside analogue of alpha-GalCer, alpha-C-galactosylceramide (alpha-C-GalCer), acts as pure killer T cell ligand in vivo, and stimulates an enhanced Th1-type response in mice.

In two illness fashions requiring Th1-type responses for management, particularly malaria and melanoma metastases, alpha-C-GalCer exhibited a 1,000-fold stronger antimalaria exercise and a 100-fold stronger antimetastatic exercise than alpha-GalCer. Moreover, alpha-C-GalCer constantly stimulated extended manufacturing of the Th1 cytokines interferon-gamma and interleukin (IL)-12, and decreased manufacturing of the Th2 cytokine IL-Four in contrast with alpha-GalCer.

Finally, alpha-C-GalCer’s enhanced therapeutic exercise required the presence of IL-12, which was wanted to stimulate pure killer cells for optimum interferon-gamma manufacturing, however didn’t have an effect on IL-4. Overall, our outcomes counsel that alpha-C-GalCer might someday be a wonderful therapeutic choice for ailments resolved by Th1-type responses.

Dividing the giant glycoside hydrolase household 13 into subfamilies: in direction of improved practical annotations of alpha-amylase-related proteins.

Family GH13, also called the alpha-amylase household, is the largest sequence-based household of glycoside hydrolases and teams collectively a quantity of totally different enzyme actions and substrate specificities appearing on alpha-glycosidic bonds. This polyspecificity ends in the undeniable fact that the easy membership of this household can’t be used for the prediction of gene perform based mostly on sequence alone.

In order to determine sturdy teams that present an improved correlation between sequence and enzymatic specificity, now we have carried out a large-scale evaluation of 1691 household GH13 sequences by combining clustering, similarity search and phylogenetic strategies. About 80% of the sequences could possibly be reliably categorised into 35 subfamilies.

Human Glycogenin 1(GYG1)ELISA Kit

QY-E01261 Qayee Biotechnology 96T
EUR 433.2

Human Glycogenin 1 (GYG1)ELISA Kit

YLA3357HU-48T Shanghai YL Biotech 48T
EUR 435

Human Glycogenin 1 (GYG1)ELISA Kit

YLA3357HU-96T Shanghai YL Biotech 96T
EUR 562.5

GYG1 Glycogenin-1 Human Recombinant Protein

PROTP46976 BosterBio Regular: 20ug
EUR 380.4
Description: GYG1 Human Recombinant fused with a 32 amino acid His-T7 tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 365 amino acids (1-333 a.a.) and having a molecular mass of 41.2kDa.;The GYG1 is purified by proprietary chromatographic techniques.

Custom Antibody titration by ELISA up to 2 rabbits and 1 bleed

ELISA-1 Alpha Diagnostics 1
EUR 242.4

Monoclonal GYG1 / Glycogenin Antibody (clone 3B5), Clone: 3B5

AMM05275G Leading Biology 0.05mg
EUR 633.6
Description: A Monoclonal antibody against Human GYG1 / Glycogenin (clone 3B5). The antibodies are raised in Mouse and are from clone 3B5. This antibody is applicable in WB and IHC-P, E

Gyg1/ Rat Gyg1 ELISA Kit

ELI-09731r Lifescience Market 96 Tests
EUR 1063.2

GYG1 Rabbit pAb

A10218-100ul Abclonal 100 ul
EUR 369.6

GYG1 Rabbit pAb

A10218-200ul Abclonal 200 ul
EUR 550.8

GYG1 Rabbit pAb

A10218-20ul Abclonal 20 ul
EUR 219.6

GYG1 Rabbit pAb

A10218-50ul Abclonal 50 ul
EUR 267.6

Glycogenin-1 (Recombinant)

20-abx073518 Abbexa
  • EUR 4519.20
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 20 ug
  • 5 ug

anti-Glycogenin 1

YF-PA23854 Abfrontier 50 ul
EUR 400.8
Description: Mouse polyclonal to Glycogenin 1

anti-Glycogenin 1

YF-PA12217 Abfrontier 50 ul
EUR 435.6
Description: Mouse polyclonal to Glycogenin 1

anti-Glycogenin 1

YF-PA12218 Abfrontier 50 ug
EUR 435.6
Description: Mouse polyclonal to Glycogenin 1

anti-Glycogenin 1

YF-PA12219 Abfrontier 100 ug
EUR 483.6
Description: Rabbit polyclonal to Glycogenin 1

GYG1 ELISA KIT|Human

EF010048 Lifescience Market 96 Tests
EUR 826.8

GYG1 Antibody

1-CSB-PA010070GA01HU Cusabio
  • EUR 716.40
  • EUR 399.60
  • 150ul
  • 50ul
Description: A polyclonal antibody against GYG1. Recognizes GYG1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB

ExoAb Antibody Kit (CD9, CD63, CD81, Hsp70 antibodies, rabbit anti-human) with goat anti-rabbit HRP secondary antibody

EXOAB-KIT-1 SBI 25 ul each
EUR 752.4

Recombinant Human Glycogenin-1

7-02920 CHI Scientific 5µg Ask for price

Recombinant Human Glycogenin-1

7-02921 CHI Scientific 20µg Ask for price

Recombinant Human Glycogenin-1

7-02922 CHI Scientific 1mg Ask for price

Anti-Glycogenin 1 (2C10)

YF-MA13392 Abfrontier 100 ug
EUR 435.6
Description: Mouse monoclonal to Glycogenin 1

Human Glycogenin 2 (GYG2)ELISA Kit

201-12-2720 SunredBio 96 tests
EUR 528
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human Glycogenin 2 (GYG2) ELISA Kit

abx387725-96tests Abbexa 96 tests
EUR 1093.2

Human Glycogenin- 2, GYG2 ELISA KIT

ELI-09732h Lifescience Market 96 Tests
EUR 988.8

Human Glycogenin 2(GYG2)ELISA Kit

QY-E01260 Qayee Biotechnology 96T
EUR 433.2

Human Glycogenin 2 (GYG2)ELISA Kit

YLA1959HU-48T Shanghai YL Biotech 48T
EUR 435

Human Glycogenin 2 (GYG2)ELISA Kit

YLA1959HU-96T Shanghai YL Biotech 96T
EUR 562.5

GYG1 siRNA

20-abx918965 Abbexa
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

GYG1 siRNA

20-abx918966 Abbexa
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

GYG1 antibody

70R-17662 Fitzgerald 50 ul
EUR 522
Description: Rabbit polyclonal GYG1 antibody

GYG1 siRNA

20-abx902383 Abbexa
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

Glycogenin 1 protein (T7 tag)

80R-1855 Fitzgerald 100 ug
EUR 366
Description: Purified recombinant Human Glycogenin 1 protein (T7 tag)

anti-Glycogenin 2

YF-PA15978 Abfrontier 50 ul
EUR 435.6
Description: Mouse polyclonal to Glycogenin 2

anti-Glycogenin 2

YF-PA15979 Abfrontier 100 ug
EUR 483.6
Description: Rabbit polyclonal to Glycogenin 2

HRP-Goat Anti-Rabbit Secondary Antibody

A12004 EpiGentek
  • EUR 232.26
  • EUR 117.70
  • 350 µg
  • 350 ug

GYG1 cloning plasmid

CSB-CL010070HU-10ug Cusabio 10ug
EUR 468
Description: A cloning plasmid for the GYG1 gene.

anti- GYG1 antibody

FNab03734 FN Test 100µg
EUR 658.5
Description: Antibody raised against GYG1

GYG1 Polyclonal Antibody

27331-100ul SAB 100ul
EUR 302.4

GYG1 Polyclonal Antibody

27331-50ul SAB 50ul
EUR 224.4

Anti-GYG1 antibody

PAab03734 Lifescience Market 100 ug
EUR 463.2

Anti-GYG1 antibody

STJ112256 St John's Laboratory 100 µl
EUR 332.4
Description: This gene encodes a member of the glycogenin family. Glycogenin is a glycosyltransferase that catalyzes the formation of a short glucose polymer from uridine diphosphate glucose in an autoglucosylation reaction. This reaction is followed by elongation and branching of the polymer, catalyzed by glycogen synthase and branching enzyme, to form glycogen. This gene is expressed in muscle and other tissues. Mutations in this gene result in glycogen storage disease XV. This gene has pseudogenes on chromosomes 1, 8 and 13 respectively. Alternatively spliced transcript variants encoding different isoforms have been identified.

Goat Anti-Rabbit Secondary Antibody, Biotin Conjugated

A12001 EpiGentek
  • EUR 232.26
  • EUR 117.70
  • 350 µg
  • 350 ug

mRNAExpress mRNA Synthesis kit (5 reactions)

MR-KIT-1 SBI 5 reactions
EUR 1382.4

Glycogenin 2 (GYG2) Antibody

20-abx116843 Abbexa
  • EUR 878.40
  • EUR 477.60
  • 150 ul
  • 50 ul

Glycogenin 2 (GYG2) Antibody

abx030300-400ul Abbexa 400 ul
EUR 627.6

Glycogenin 2 (GYG2) Antibody

abx030300-80l Abbexa 80 µl
EUR 343.2

Glycogenin 2 (GYG2) Antibody

abx036618-100ug Abbexa 100 ug
EUR 469.2

Glycogenin 2 (GYG2) Antibody

abx233735-100ug Abbexa 100 ug
EUR 661.2

Anti-Glycogenin 2 (3D10)

YF-MA16601 Abfrontier 100 ug
EUR 435.6
Description: Mouse monoclonal to Glycogenin 2

PinPoint-FC 293T Platform Kit for Targeted Gene Insertion (includes PIN320A-1, PIN200A-1, PIN510A-1 & PIN600A-1)

PIN320A-KIT SBI 1 Kit
EUR 5929.2

PinPoint-FC Murine iPSC Platform Kit for Targeted Gene Insertion (includes PIN340iPS-1, PIN200A-1, PIN510A-1 & PIN600A-1)

PIN340iPS-KIT SBI 1 Kit
EUR 5929.2

Rabbit Albumin AssayMax ELISA Kit

ETA2202-1 AssayPro 96 Well Plate
EUR 475.2

GYG1 Polyclonal Conjugated Antibody

C27331 SAB 100ul
EUR 476.4

Human GYG1 shRNA Plasmid

20-abx952027 Abbexa
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

Mouse GYG1 shRNA Plasmid

20-abx973868 Abbexa
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

Rat GYG1 shRNA Plasmid

20-abx986645 Abbexa
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

GYG1 Recombinant Protein (Rat)

RP204065 ABM 100 ug Ask for price

GYG1 Recombinant Protein (Human)

RP014278 ABM 100 ug Ask for price

T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents)

CAS510A-KIT SBI 1 Kit
EUR 966

PinPoint-FC System for Platform Cell Line Generation & Retargeting (includes PIN300A-1, FC200PA-1, PIN200A-1, PIN510A-1, & PIN600A-1)

PIN300A-KIT SBI 1 Kit
EUR 3357.6

PinPoint-HR System for Platform Cell Line Generation & Retargeting (includes PIN400A-1, PIN200A-1, PIN510A-1, & PIN600A-1)

PIN400A-KIT SBI 1 Kit
EUR 3357.6

Rabbit Albumin AssayMax ELISA Kit (Discontinued, replaced by ETA2202-1)

ETA2201-1 AssayPro 96 Well Plate
EUR 475.2

GYG1 sgRNA CRISPR Lentivector (Human) (Target 1)

K0922702 ABM 1.0 ug DNA
EUR 184.8

Gyg1 sgRNA CRISPR Lentivector (Rat) (Target 1)

K6973602 ABM 1.0 ug DNA
EUR 184.8

PinPoint-HR System for Platform Cell Line Generation & Retargeting of AAVS1 Safe Harbor Locus (includes PIN410A-1, GE601A-1, PIN200A-1, PIN510A-1, & PIN600A-1)

PIN410A-KIT SBI 1 Kit
EUR 5202

PinPoint-HR System for Platform Cell Line Generation & Retargeting of AAVS1 Safe Harbor Locus (includes PIN410A-1, CAS601A-1, PIN200A-1, PIN510A-1, & PIN600A-1)

PIN412A-KIT SBI 1 Kit
EUR 5202

AXYPET STARTER KIT 1 AP-20, AP-200 & AP-1000 WITH ADDITIONAL FREE RACKS OF AXYGEN PIPETTE TIPS

AP-STR-KIT-1 CORNING 1/pk
EUR 426
Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller

Frit Kit

FRIT-KIT Next Advance 1each
EUR 148.8
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool.

Column Packing Kit

PACK-KIT Next Advance 1pack
EUR 1242
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar.

Assurance Grade Set of 38 Single-Element Standards 1000 ug/mL (1000 PPM)

ICP-KIT-1 Scientific Laboratory Supplies EACH
EUR 1914.06

Gyg1 ORF Vector (Rat) (pORF)

ORF068023 ABM 1.0 ug DNA
EUR 607.2

GYG1 ORF Vector (Human) (pORF)

ORF004760 ABM 1.0 ug DNA
EUR 114

h GYG1 inducible lentiviral particles

LVP415 GenTarget 1 x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made optional inducible lentiviral particles for expressing human target: h GYG1 (alternative name: GYG; GSD15), with ORF sequence 100% matching to CDS region in NCBI ID: NM_004130.3. Particles also contains a RFP-Blasticidin dual selection marker.

PCR Mycoplasma Detection Kit

M034-Kit TOKU-E Kit
EUR 319.2

AAVS1 Safe Harbor Targeting Vector 2.0 - All-Purpose Donor (AAVS1-SA-puro-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)

GE620A-KIT SBI 1 kit
EUR 2558.4

AAVS1 Safe Harbor Targeting Vector 2.0 - GOI Knock-in Donor (AAVS1-SA-puro-EF1-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)

GE622A-KIT SBI 1 kit
EUR 2558.4

AAVS1 Safe Harbor Targeting Vector 2.0 - Reporter Knock-in Donor (AAVS1-SA-puro-MCS-GFP), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)

GE624A-KIT SBI 1 kit
EUR 2558.4

Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit

CAS400A-KIT SBI 1 kit (10 rxn)
EUR 1332

Pesticide Kit Containing All 10 Multi-Compound Mixes

SPXPR-KIT Scientific Laboratory Supplies 1ML
EUR 1923.18

Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit

CAS410A-KIT SBI 1 kit (10 rxn)
EUR 798

Rabbit Interleukin 1, IL-1 ELISA Kit

1-CSB-E06896Rb Cusabio
  • EUR 723.60
  • EUR 5096.40
  • EUR 2712.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Rabbit Interleukin 1, IL-1 in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Rabbit Endothelin 1, ET-1 ELISA Kit

1-CSB-E06951Rb Cusabio
  • EUR 1160.40
  • EUR 7110.00
  • EUR 3760.80
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Rabbit Endothelin 1, ET-1 in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Cas9-EGFP Protein and T7 gRNA SmartNuclease Synthesis Kit

CAS420A-KIT SBI 1 kit (10 rxn)
EUR 828

CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV100PA-KIT SBI 1 Kit
EUR 1358.4

CMV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV105PA-KIT SBI 1 Kit
EUR 1358.4

MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV120PA-KIT SBI 1 Kit
EUR 1358.4

MSCV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV125PA-KIT SBI 1 Kit
EUR 1358.4

Multiplex gRNA Kit + EF1-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS700A-KIT SBI 10 rxn
EUR 1358.4

Multiplex gRNA Kit + CAG-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS720A-KIT SBI 10 rxn
EUR 1358.4

Multiplex gRNA Kit + CMV-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS740A-KIT SBI 10 rxn
EUR 1358.4

Rabbit Dickkopf 1(DKK1)ELISA Kit

1-CSB-E16508Rb Cusabio
  • EUR 1080.00
  • EUR 6571.20
  • EUR 3480.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Rabbit Dickkopf 1(DKK1) in samples from serum, plasma. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Rabbit paraoxonase 1 (PON1) ELISA kit

1-CSB-EL018369RB Cusabio
  • EUR 1080.00
  • EUR 6571.20
  • EUR 3480.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Rabbit paraoxonase 1 (PON1) in samples from serum, plasma, tissue homogenates, cell lysates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

GYG1 sgRNA CRISPR Lentivector set (Human)

K0922701 ABM 3 x 1.0 ug
EUR 406.8

Gyg1 sgRNA CRISPR Lentivector set (Rat)

K6973601 ABM 3 x 1.0 ug
EUR 406.8

EpiQuik Nuclear Extraction Kit 

OP-0002 EpiGentek
  • EUR 350.58
  • EUR 211.20
  • 100 Assays
  • 100 Extractions

Cas9 Nickase: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV200PA-KIT SBI 1 Kit
EUR 1358.4

Cas9 Nickase: CMV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV205PA-KIT SBI 1 Kit
EUR 1358.4

Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV220PA-KIT SBI 1 Kit
EUR 1358.4

Cas9 Nickase: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV225PA-KIT SBI 1 Kit
EUR 1358.4

Multiplex gRNA Kit + Cas9 Nickase: EF1-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS750A-KIT SBI 10 rxn
EUR 1358.4

Multiplex gRNA Kit + Cas9 Nickase: CAG-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS770A-KIT SBI 10 rxn
EUR 1358.4

Multiplex gRNA Kit + Cas9 Nickase: CMV-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS790A-KIT SBI 10 rxn
EUR 1358.4

Methylamp 96 DNA Modification Kit 

P-1008 EpiGentek
  • EUR 416.70
  • EUR 253.00
  • 96 Samples
  • 96 Samples

Cas9 SmartNuclease Extra Ligation Kit [includes 5x ligation buffer (10 ul) and Fast ligase (2.5ul)]

CAS9LIG-KIT SBI 1 Kit
EUR 183.6

Claritas PPT Grade Minerals Testing Kit for ICP-MS 1000 ug/mL (1000 ppm) 6 each 30mL

SPXMT-KIT Scientific Laboratory Supplies 30ML
EUR 237.12

Rabbit matrix metalloproteinase 1, MMP-1 ELISA kit

1-CSB-E09744Rb Cusabio
  • EUR 722.40
  • EUR 5085.60
  • EUR 2707.20
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Rabbit matrix metalloproteinase 1, MMP-1 in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Claritas PPT Grade Heavy Metals Testing Kit for ICP-MS 1000 ug/mL (1000 ppm) 6 each 30mL

SPXHM-KIT Scientific Laboratory Supplies 30ML
EUR 245.1

Rabbit MUSCLE ACETONE POWDER (1-g)

41995-1 Pel-Freez 1g
EUR 144.55

Rabbit SCLERA YG FROZEN 1 EA

41216-1 Pel-Freez 1 Each
EUR 100.19

Anti-Sumo 1 Rabbit Monoclonal Antibody

M00631-1 BosterBio 100ug/vial
EUR 476.4
Description: Rabbit Monoclonal Sumo 1 Antibody. Validated in IP, IF, IHC, WB and tested in Human, Mouse, Rat.

Anti-Cytokeratin 1 Rabbit Monoclonal Antibody

M01639-1 BosterBio 100ug/vial
EUR 476.4
Description: Rabbit Monoclonal Cytokeratin 1 Antibody. Validated in WB and tested in Human, Mouse, Rat.

pPACK-SPIKE Combo Kit, includes Cat# CVD19-500A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)

CVD19-520A-KIT SBI 1 kit
EUR 1141

Human Hexokinase-1 AssayMax ELISA Kit

EH3101-1 AssayPro 96 Well Plate
EUR 572.4

Rat TIMP-1 AssayMax ELISA Kit

ERT2538-1 AssayPro 96 Well Plate
EUR 572.4

Human Glutaredoxin-1 AssayMax ELISA Kit

EG2153-1 AssayPro 96 Well Plate
EUR 500.4

Human Complexin-1 AssayMax ELISA Kit

EC3505-1 AssayPro 96 Well Plate
EUR 500.4

pPACK-SPIKE D614G Combo Kit, includes Cat# CVD19-530A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)

CVD19-550A-KIT SBI 1 kit
EUR 1141

pPACK-SPIKE N501Y Combo Kit, includes Cat# CVD19-560A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)

CVD19-575A-KIT SBI 1 kit
EUR 1141

pPACK-SPIKE Alpha Combo Kit, includes Cat# CVD19-590A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)

CVD19-599A-KIT SBI 1 kit
EUR 1141

pPACK-SPIKE Gamma Combo Kit, includes Cat# CVD19-630A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)

CVD19-639A-KIT SBI 1 kit
EUR 1141

pPACK-SPIKE Beta Combo Kit, includes Cat# CVD19-640A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)

CVD19-649A-KIT SBI 1 kit
EUR 1141

pPACK-SPIKE Delta Combo Kit, includes Cat# CVD19-650A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)

CVD19-659A-KIT SBI 1 kit
EUR 1141

pPACK-SPIKE Omicron Combo Kit, includes Cat# CVD19-660A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)

CVD19-669A-KIT SBI 1 kit
EUR 1162

pPACK-SPIKE B.1.429 Combo Kit, includes Cat# CVD19-610A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)

CVD19-619A-KIT SBI 1 kit
EUR 1141

pPACK-SPIKE B.1.525 Combo Kit, includes Cat# CVD19-620A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)

CVD19-629A-KIT SBI 1 kit
EUR 1141

pPACK-SPIKE B.1.351 RBD Mutations Combo Kit, includes Cat# CVD19-580A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)

CVD19-589A-KIT SBI 1 kit
EUR 1141

pPACK-SPIKE B.1.351 S1 Mutations Combo Kit, includes Cat# CVD19-600A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)

CVD19-609A-KIT SBI 1 kit
EUR 1141

Anti-c-Kit Rabbit Monoclonal Antibody

M01335-1 BosterBio 100ug/vial
EUR 476.4
Description: Rabbit Monoclonal c-Kit Antibody. Validated in IHC, WB and tested in Human.

Rabbit PSOAS MUSCLE YG FROZEN 1 EA

41217-1 Pel-Freez 1 Each
EUR 216.34

Anti-Integrin beta 1 Rabbit Monoclonal Antibody

M00772-1 BosterBio 100ug/vial
EUR 476.4
Description: Rabbit Monoclonal Integrin beta 1 Antibody. Validated in IHC, WB and tested in Human, Mouse, Rat.

Anti-ARG1/Arginase 1 Rabbit Monoclonal Antibody

M01106-1 BosterBio 100ug/vial
EUR 476.4
Description: Rabbit Monoclonal ARG1/Arginase 1 Antibody. Validated in IP, WB and tested in Human, Mouse, Rat.

Anti-MLANA/Mart 1 Rabbit Monoclonal Antibody

M02033-1 BosterBio 100ug/vial
EUR 476.4
Description: Rabbit Monoclonal MLANA/Mart 1 Antibody. Validated in IF, WB and tested in Human.

Anti-DSG1/Desmoglein 1 Rabbit Monoclonal Antibody

M02655-1 BosterBio 100ug/vial
EUR 476.4
Description: Rabbit Monoclonal DSG1/Desmoglein 1 Antibody. Validated in IF, IHC, ICC, WB and tested in Human, Mouse, Rat.

Anti-HMGB1/Hmg 1 Rabbit Monoclonal Antibody

M00066-1 BosterBio 100ug/vial
EUR 476.4
Description: Rabbit Monoclonal HMGB1/Hmg 1 Antibody. Validated in Flow Cytometry, IF, IHC, ICC, WB and tested in Human, Mouse, Rat.

Anti-p27 KIP 1 Rabbit Monoclonal Antibody

M00173-1 BosterBio 100ug/vial
EUR 476.4
Description: Anti-p27 KIP 1 Rabbit Monoclonal Antibody tested for Flow Cytometry, IP, IF, IHC, ICC, WB in Human, Rat

Anti-MHC class 1 Rabbit Monoclonal Antibody

M00194-1 BosterBio 100ug/vial
EUR 476.4
Description: Rabbit Monoclonal MHC class 1 Antibody. Validated in IP, IF, IHC, WB and tested in Human.

Monoclonal GYG1 Antibody (monoclonal) (M07), Clone: 3B5

AMM05276G Leading Biology 0.1mg
EUR 580.8
Description: A Monoclonal antibody against Human GYG1 (monoclonal) (M07). The antibodies are raised in mouse and are from clone 3B5. This antibody is applicable in WB and IHC, E

Monoclonal GYG1 Antibody (monoclonal) (M08), Clone: 2C10

AMM05277G Leading Biology 0.1mg
EUR 580.8
Description: A Monoclonal antibody against Human GYG1 (monoclonal) (M08). The antibodies are raised in mouse and are from clone 2C10. This antibody is applicable in WB

GYG1 sgRNA CRISPR Lentivector (Human) (Target 2)

K0922703 ABM 1.0 ug DNA
EUR 184.8

GYG1 sgRNA CRISPR Lentivector (Human) (Target 3)

K0922704 ABM 1.0 ug DNA
EUR 184.8

Gyg1 sgRNA CRISPR Lentivector (Rat) (Target 2)

K6973603 ABM 1.0 ug DNA
EUR 184.8

Gyg1 sgRNA CRISPR Lentivector (Rat) (Target 3)

K6973604 ABM 1.0 ug DNA
EUR 184.8

GYG1 Protein Vector (Human) (pPB-C-His)

PV019037 ABM 500 ng
EUR 394.8

GYG1 Protein Vector (Human) (pPB-N-His)

PV019038 ABM 500 ng
EUR 394.8

GYG1 Protein Vector (Human) (pPM-C-HA)

PV019039 ABM 500 ng
EUR 394.8

GYG1 Protein Vector (Human) (pPM-C-His)

PV019040 ABM 500 ng
EUR 394.8

Recombinant Human GYG1 Protein, Untagged, E.coli-1mg

QP12070-1mg EnQuireBio 1mg
EUR 3667.2

Recombinant Human GYG1 Protein, Untagged, E.coli-20ug

QP12070-20ug EnQuireBio 20ug
EUR 241.2

Recombinant Human GYG1 Protein, Untagged, E.coli-5ug

QP12070-5ug EnQuireBio 5ug
EUR 186

GYG1 Protein Vector (Rat) (pPB-C-His)

PV272090 ABM 500 ng
EUR 723.6

GYG1 Protein Vector (Rat) (pPB-N-His)

PV272091 ABM 500 ng
EUR 723.6

GYG1 Protein Vector (Rat) (pPM-C-HA)

PV272092 ABM 500 ng
EUR 723.6

GYG1 Protein Vector (Rat) (pPM-C-His)

PV272093 ABM 500 ng
EUR 723.6

Gyg1 3'UTR Luciferase Stable Cell Line

TU205590 ABM 1.0 ml Ask for price

Gyg1 3'UTR GFP Stable Cell Line

TU255590 ABM 1.0 ml Ask for price

GYG1 3'UTR GFP Stable Cell Line

TU059484 ABM 1.0 ml
EUR 1672.8

GYG1 3'UTR Luciferase Stable Cell Line

TU009484 ABM 1.0 ml
EUR 1672.8

Rabbit plasminogen activator inhibitor 1, PAI-1 ELISA Kit

1-CSB-E06978Rb Cusabio
  • EUR 1160.40
  • EUR 7110.00
  • EUR 3760.80
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativecompetitive ELISA kit for measuring Rabbit plasminogen activator inhibitor 1, PAI-1 in samples from serum, plasma, cell culture supernates, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Rabbit Alpha-1-acid glycoprotein 1(ORM1) ELISA kit

1-CSB-EL017237RB Cusabio
  • EUR 843.60
  • EUR 5811.60
  • EUR 3084.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Rabbit Alpha-1-acid glycoprotein 1(ORM1) in samples from serum, plasma, cell culture supernates, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Human TGF-beta-1 AssayMax ELISA Kit

ET3102-1 AssayPro 96 Well Plate
EUR 572.4

Human PAI-1/tPA AssayMax ELISA Kit

EP1105-1 AssayPro 96 Well Plate
EUR 500.4

Human Lipocalin-1 (LCN1) AssayMax ELISA Kit

EL3502-1 AssayPro 96 Well Plate
EUR 572.4

Anti-BOB-1/OBF-1 Rabbit Monoclonal Antibody, Clone#RM378

M04431-1 BosterBio 100uL
EUR 462
Description: Anti-BOB-1/OBF-1 Rabbit Monoclonal Antibody, Clone#RM378 tested in WB, IHC, reactive to Human

Mouse Interleukin-1 beta (IL-1 beta) AssayMax ELISA Kit

EMI2200-1 AssayPro 96 Well Plate
EUR 572.4

Human Interleukin-1 beta (IL-1 beta) AssayMax ELISA Kit

EI2200-1 AssayPro 96 Well Plate
EUR 572.4

Human Interleukin-1-alpha (IL-1-alpha) AssayMax ELISA Kit

EI2301-1 AssayPro 96 Well Plate
EUR 572.4

Human KRAB-associated Protein 1 (KAP-1) AssayMax ELISA Kit

EK2802-1 AssayPro 96 Well Plate
EUR 572.4

Human Plasminogen Activator Inhibitor-1 (PAI-1) AssayMax ELISA Kit

EP1100-1 AssayPro 96 Well Plate
EUR 500.4

Histone H3 Methylation Antibody Panel Pack I – Active Genes

C10000 EpiGentek
  • EUR 928.26
  • EUR 588.50
  • 4 x 25 µg
  • 4 x 25 ul

Histone H3 Methylation Antibody Panel Pack I – Repression Genes

C10001 EpiGentek
  • EUR 928.26
  • EUR 588.50
  • 4 x 25 µg
  • 4 x 25 ul

Histone H3 Methylation Antibody Panel Pack II – Active Genes

C10002 EpiGentek
  • EUR 754.26
  • EUR 470.80
  • 3 x 25 µg
  • 3 x 25 ul

Histone H3 Methylation Antibody Panel Pack II – Repression Genes

C10003 EpiGentek
  • EUR 928.26
  • EUR 588.50
  • 4 x 25 µg
  • 4 x 25 ul

Histone H3 Methylation Antibody Panel Pack III – Active Genes

C10004 EpiGentek
  • EUR 928.26
  • EUR 588.50
  • 4 x 25 µg
  • 4 x 25 ul

Histone H3K4 Methylation Antibody Panel Pack

C10005 EpiGentek
  • EUR 754.26
  • EUR 470.80
  • 3 x 25 µg
  • 3 x 25 ul

Histone H3K9 Methylation Antibody Panel Pack

C10006 EpiGentek
  • EUR 754.26
  • EUR 470.80
  • 3 x 25 µg
  • 3 x 25 ul

Histone H3K27 Methylation Antibody Panel Pack

C10007 EpiGentek
  • EUR 754.26
  • EUR 470.80
  • 3 x 25 µg
  • 3 x 25 ul

Histone H3K36 Methylation Antibody Panel Pack

C10008 EpiGentek
  • EUR 754.26
  • EUR 470.80
  • 3 x 25 µg
  • 3 x 25 ul

Histone H3K79 Methylation Antibody Panel Pack

C10009 EpiGentek
  • EUR 754.26
  • EUR 470.80
  • 3 x 25 µg
  • 3 x 25 ul

Histone H3 Acetylation Antibody Panel Pack I

C10010 EpiGentek
  • EUR 928.26
  • EUR 588.50
  • 4 x 25 µg
  • 4 x 25 ul

Histone H3 Acetylation Antibody Panel Pack II

C10011 EpiGentek
  • EUR 928.26
  • EUR 588.50
  • 4 x 25 µg
  • 4 x 25 ul

Histone H4K20 Methylation Antibody Panel Pack

C10012 EpiGentek
  • EUR 754.26
  • EUR 470.80
  • 3 x 25 µg
  • 3 x 25 ul

Histone H4 Acetylation Antibody Panel Pack

C10013 EpiGentek
  • EUR 754.26
  • EUR 470.80
  • 4 x 25 µg
  • 4 x 25 ul

Histone H3 Phosphorylation Antibody Panel Pack

C10014 EpiGentek
  • EUR 754.26
  • EUR 470.80
  • 3 x 25 µg
  • 3 x 25 ul

Histone H3R2 Methylation Antibody Panel Pack

C10015 EpiGentek
  • EUR 754.26
  • EUR 470.80
  • 3 x 25 µg
  • 3 x 25 ul

Histone H3R8 Methylation Antibody Panel Pack

C10016 EpiGentek
  • EUR 754.26
  • EUR 470.80
  • 3 x 25 µg
  • 3 x 25 ul

Histone H3R17 Methylation Antibody Panel Pack

C10017 EpiGentek
  • EUR 754.26
  • EUR 470.80
  • 3 x 25 µg
  • 3 x 25 ul

Histone H3R26 Methylation Antibody Panel Pack

C10018 EpiGentek
  • EUR 754.26
  • EUR 470.80
  • 3 x 25 µg
  • 3 x 25 ul

Histone H4R3 Methylation Antibody Panel Pack

C10019 EpiGentek
  • EUR 754.26
  • EUR 470.80
  • 3 x 25 µg
  • 3 x 25 ul

DNMT3A Protein

E11000 EpiGentek
  • EUR 971.76
  • EUR 618.20
  • 10 µg
  • 10 ul

TET1 Protein (Active)

E12002 EpiGentek
  • EUR 387.12
  • EUR 225.50
  • 10 µg
  • 10 ul

DNMT3B Protein 

E14000 EpiGentek
  • EUR 971.76
  • EUR 618.20
  • 10 µg
  • 10 ul

DNMT1 Protein 

E15000 EpiGentek
  • EUR 971.76
  • EUR 618.20
  • 10 µg
  • 10 ul

HDAC1 Protein 

E24002 EpiGentek
  • EUR 968.28
  • EUR 616.00
  • 50 µg
  • 50 ul

HDAC10 Protein 

E24003 EpiGentek
  • EUR 968.28
  • EUR 616.00
  • 50 µg
  • 50 ul

HDAC11 Protein 

E24004 EpiGentek
  • EUR 968.28
  • EUR 616.00
  • 50 µg
  • 50 ul

HDAC2 Protein 

E24005 EpiGentek
  • EUR 968.28
  • EUR 616.00
  • 50 µg
  • 50 ul

HDAC3 Protein 

E24006 EpiGentek
  • EUR 968.28
  • EUR 616.00
  • 50 µg
  • 50 ul

HDAC4 Protein 

E24007 EpiGentek
  • EUR 968.28
  • EUR 616.00
  • 10 µg
  • 10 ul

HDAC5 Protein 

E24008 EpiGentek
  • EUR 968.28
  • EUR 616.00
  • 10 µg
  • 10 ul

HDAC6 Protein 

E24009 EpiGentek
  • EUR 968.28
  • EUR 616.00
  • 50 µg
  • 50 ul

HDAC7 Protein 

E24010 EpiGentek
  • EUR 968.28
  • EUR 616.00
  • 10 µg
  • 10 ul

HDAC8 Protein 

E24011 EpiGentek
  • EUR 968.28
  • EUR 616.00
  • 50 µg
  • 50 ul

HDAC9 Protein 

E24012 EpiGentek
  • EUR 968.28
  • EUR 616.00
  • 10 µg
  • 10 ul

H3F3A Protein 

E35003 EpiGentek
  • EUR 174.84
  • EUR 78.10
  • 50 µg
  • 50 ul

H4 Protein 

E35005 EpiGentek
  • EUR 174.84
  • EUR 78.10
  • 50 µg
  • 50 ul

SOD1 Protein 

E70000 EpiGentek
  • EUR 599.40
  • EUR 365.20
  • 25 µg
  • 25 ul

Goat Anti-Rat Secondary Antibody, Biotin Conjugated

A12002 EpiGentek
  • EUR 232.26
  • EUR 117.70
  • 350 µg
  • 350 ug

HRP-Goat Anti-Mouse Secondary Antibody

A12003 EpiGentek
  • EUR 232.26
  • EUR 117.70
  • 350 µg
  • 350 ug

EpiMag HT (96-Well) Magnetic Separator

Q10002 EpiGentek
  • EUR 416.70
  • EUR 258.50
  • 1/Pack
  • 1/Pack

EpiMag 96-Well Microplate (5/pack) 

Q10003 EpiGentek
  • EUR 136.56
  • EUR 52.80
  • 5/pack
  • 5/pack

Human Genomic DNA 

X11000 EpiGentek
  • Ask for price
  • EUR 235.40
  • 0.2 ml
  • 0.2 ml

Human Brain Genomic DNA  

X11001 EpiGentek
  • Ask for price
  • EUR 77.00
  • 10 µg
  • 10 ul

DNA Methylation Antibody Panel Pack I

C20000 EpiGentek
  • EUR 458.46
  • EUR 270.60
  • each
  • 2 x 25 ul
×

Most subfamilies seem monofunctional (i.e. include enzymes with the similar substrate and the similar product). The shut examination of the different, apparently polyspecific, subfamilies revealed that they really group collectively enzymes with strongly associated (and even generally nearly equivalent) actions. Overall our subfamily task permits to set the limits for genomic perform prediction on this huge household of biologically and industrially vital enzymes.

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Recent Posts

  • Compare polyclonal lab reagents for research
  • Compare polyclonal lab reagents for research
  • Antibody
  • cDNA
  • DNA
  • Elisa Kits
  • Hamster
  • HEPES
  • Heterologous expression and mutagenesis of recombinant Vespa affinis hyaluronidase protein (rVesA2).
  • high
  • Horse
  • Horse serum
  • host
  • HRP
  • In Planta Glycan Engineering and Functional Activities of IgE Antibodies.
  • phosphor
  • reverse
  • RIA
  • rnai
  • TEMED
  • The mucin-selective protease StcE enables molecular and functional analysis of human cancer-associated mucins.
  • Tomato UDP-Glucose Sterol Glycosyltransferases: A Family of Developmental and Stress Regulated Genes that Encode Cytosolic and Membrane-Associated Forms of the Enzyme.
  • Two Novel Fungal Phenolic UDP Glycosyltransferases from Absidia coerulea and Rhizopus japonicus.
  • UGT85A84 Catalyzes the Glycosylation of Aromatic Monoterpenes in Osmanthus fragrans Lour. Flowers.
  • Compare polyclonal lab reagents for research
  • Compare polyclonal lab reagents for research
  • Assay Lab Reagents for Research
  • Gene-centric metagenomics of the fiber-adherent bovine rumen microbiome reveals forage specific glycoside hydrolases.
  • Stimulation of lignocellulosic biomass hydrolysis by proteins of glycoside hydrolase family 61: structure and function of a large, enigmatic family.
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