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Gene-centric metagenomics of the fiber-adherent bovine rumen microbiome reveals forage specific glycoside hydrolases.

Gene-centric metagenomics of the fiber-adherent bovine rumen microbiome reveals forage specific glycoside hydrolases.

Posted on September 25, 2021July 25, 2021 by Mathew

The complicated microbiome of the rumen capabilities as an efficient system for the conversion of plant cell wall biomass to microbial protein, quick chain fatty acids, and gases. As such, it supplies a singular genetic useful resource for plant cell wall degrading microbial enzymes that could possibly be utilized in the manufacturing of biofuels. The rumen and gastrointestinal tract harbor a dense and complicated microbiome.

To achieve a larger understanding of the ecology and metabolic potential of this microbiome, we used comparative metagenomics (phylotype evaluation and SEED subsystems-based annotations) to look at randomly sampled pyrosequence knowledge from 3 fiber-adherent microbiomes and 1 pooled liquid pattern (a mix of the liquid microbiome fractions from the similar bovine rumens). Even although the Three animals have been fed the similar food plan, the group construction, predicted phylotype, and metabolic potentials in the rumen have been markedly totally different with respect to nutrient utilization.

A comparability of the glycoside hydrolase and cellulosome practical genes revealed that in the rumen microbiome, preliminary colonization of fiber seems to be by organisms possessing enzymes that assault the simply obtainable facet chains of complicated plant polysaccharides and never the extra recalcitrant most important chains, particularly cellulose.

Furthermore, in comparison with the termite hindgut microbiome, there are basic variations in the glycoside hydrolase content material that look like food plan pushed for both the bovine rumen (forages and legumes) or the termite hindgut (wooden).

Digoxin and different cardiac glycosides inhibit HIF-1alpha synthesis and block tumor development.

A library of medicine which can be in scientific trials or use was screened for inhibitors of hypoxia-inducible issue 1 (HIF-1). Twenty medicine inhibited HIF-1-dependent gene transcription by >88% at a focus of 0.Four microM. Eleven of these medicine have been cardiac glycosides, together with digoxin, ouabain, and proscillaridin A, which inhibited HIF-1alpha protein synthesis and expression of HIF-1 goal genes in most cancers cells.

Digoxin administration elevated latency and decreased development of tumor xenografts, whereas remedy of established tumors resulted in development arrest inside one week. Enforced expression of HIF-1alpha by transfection was not inhibited by digoxin, and xenografts derived from these cells have been immune to the anti-tumor results of digoxin, demonstrating that HIF-1 is a important goal of digoxin for most cancers remedy.

Gene-centric metagenomics of the fiber-adherent bovine rumen microbiome reveals forage specific glycoside hydrolases.

Cardiac glycosides are a various household of naturally derived compounds that bind to and inhibit Na+/Ok+-ATPase. Members of this household have been in scientific use for a few years for the remedy of coronary heart failure and atrial arrhythmia, and the mechanism of their constructive inotropic impact is nicely characterised. Exciting latest findings have prompt further signalling modes of motion of Na+/Ok+-ATPase, implicating cardiac glycosides in the regulation of a number of vital mobile processes and highlighting potential new therapeutic roles for these compounds in numerous ailments.

Perhaps most notably, the elevated susceptibility of most cancers cells to those compounds helps their potential use as most cancers therapies, and the first technology of glycoside-based anticancer medicine are presently in scientific trials.

Superior safety in opposition to malaria and melanoma metastases by a C-glycoside analogue of the pure killer T cell ligand alpha-Galactosylceramide.

alpha-Galactosylceramide (alpha-GalCer) is a glycolipid that stimulates pure killer T cells to supply each T helper (Th) 1 and Th2 cytokines. This property permits alpha-GalCer to ameliorate all kinds of infectious, neoplastic, and autoimmune ailments; nonetheless, its effectiveness in opposition to anybody illness is restricted by the opposing actions of the induced Th1 and Th2 cytokines. Here, we report {that a} artificial C-glycoside analogue of alpha-GalCer, alpha-C-galactosylceramide (alpha-C-GalCer), acts as pure killer T cell ligand in vivo, and stimulates an enhanced Th1-type response in mice.

In two illness fashions requiring Th1-type responses for management, particularly malaria and melanoma metastases, alpha-C-GalCer exhibited a 1,000-fold stronger antimalaria exercise and a 100-fold stronger antimetastatic exercise than alpha-GalCer. Moreover, alpha-C-GalCer constantly stimulated extended manufacturing of the Th1 cytokines interferon-gamma and interleukin (IL)-12, and decreased manufacturing of the Th2 cytokine IL-Four in contrast with alpha-GalCer.

Finally, alpha-C-GalCer’s enhanced therapeutic exercise required the presence of IL-12, which was wanted to stimulate pure killer cells for optimum interferon-gamma manufacturing, however didn’t have an effect on IL-4. Overall, our outcomes counsel that alpha-C-GalCer might someday be a wonderful therapeutic choice for ailments resolved by Th1-type responses.

Dividing the giant glycoside hydrolase household 13 into subfamilies: in direction of improved practical annotations of alpha-amylase-related proteins.

Family GH13, also called the alpha-amylase household, is the largest sequence-based household of glycoside hydrolases and teams collectively a quantity of totally different enzyme actions and substrate specificities appearing on alpha-glycosidic bonds. This polyspecificity ends in the undeniable fact that the easy membership of this household can’t be used for the prediction of gene perform based mostly on sequence alone.

In order to determine sturdy teams that present an improved correlation between sequence and enzymatic specificity, now we have carried out a large-scale evaluation of 1691 household GH13 sequences by combining clustering, similarity search and phylogenetic strategies. About 80% of the sequences could possibly be reliably categorised into 35 subfamilies.

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EUR 3990

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EUR 920

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EUR 3990

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GYG1 (Glycogenin 1, Glycogenin-1, GN-1, GN1, GYG) (MaxLight 550)

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EUR 3990

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EUR 920

GYG1 (Glycogenin 1, Glycogenin-1, GN-1, GN1, GYG) (MaxLight 650)

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EUR 3990

GYG1 (Glycogenin 1, Glycogenin-1, GN-1, GN1, GYG) (MaxLight 750)

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EUR 920

GYG1 (Glycogenin 1, Glycogenin-1, GN-1, GN1, GYG) (MaxLight 750)

MBS6380658-5x01mL MyBiosource 5x0.1mL
EUR 3990

GYG1 (Glycogenin 1, Glycogenin-1, GN-1, GN1, GYG) (MaxLight 650)

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EUR 875

GYG1 (Glycogenin 1, Glycogenin-1, GN-1, GN1, GYG) (MaxLight 650)

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EUR 3800

GYG1 (Glycogenin 1, Glycogenin-1, GN-1, GN1, GYG) (MaxLight 490)

MBS6201143-01mL MyBiosource 0.1(mL
EUR 875

GYG1 (Glycogenin 1, Glycogenin-1, GN-1, GN1, GYG) (MaxLight 490)

MBS6201143-5x01mL MyBiosource 5x0.1mL
EUR 3800

GYG1 (Glycogenin 1, Glycogenin-1, GN-1, GN1, GYG) (MaxLight 405)

MBS6190468-01mL MyBiosource 0.1(mL
EUR 875

GYG1 (Glycogenin 1, Glycogenin-1, GN-1, GN1, GYG) (MaxLight 405)

MBS6190468-5x01mL MyBiosource 5x0.1mL
EUR 3800

GYG1 (Glycogenin 1, Glycogenin-1, GN-1, GN1, GYG) (MaxLight 550)

MBS6211818-01mL MyBiosource 0.1(mL
EUR 875

GYG1 (Glycogenin 1, Glycogenin-1, GN-1, GN1, GYG) (MaxLight 550)

MBS6211818-5x01mL MyBiosource 5x0.1mL
EUR 3800

GYG1 (Glycogenin 1, Glycogenin-1, GN-1, GN1, GYG) (MaxLight 750)

MBS6233168-01mL MyBiosource 0.1(mL
EUR 875

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MBS6233168-5x01mL MyBiosource 5x0.1mL
EUR 3800

Recombinant Mouse Glycogenin-1 (Gyg1)

MBS1374296-002mgBaculovirus MyBiosource 0.02mg(Baculovirus)
EUR 1210

Recombinant Mouse Glycogenin-1 (Gyg1)

MBS1374296-002mgEColi MyBiosource 0.02mg(E-Coli)
EUR 860

Recombinant Mouse Glycogenin-1 (Gyg1)

MBS1374296-002mgYeast MyBiosource 0.02mg(Yeast)
EUR 1000

Recombinant Mouse Glycogenin-1 (Gyg1)

MBS1374296-01mgEColi MyBiosource 0.1mg(E-Coli)
EUR 1035

Recombinant Mouse Glycogenin-1 (Gyg1)

MBS1374296-01mgYeast MyBiosource 0.1mg(Yeast)
EUR 1135

Recombinant Human Glycogenin-1 (GYG1)

MBS966826-002mgBaculovirus MyBiosource 0.02mg(Baculovirus)
EUR 1225

Recombinant Human Glycogenin-1 (GYG1)

MBS966826-002mgEColi MyBiosource 0.02mg(E-Coli)
EUR 880

Recombinant Human Glycogenin-1 (GYG1)

MBS966826-002mgYeast MyBiosource 0.02mg(Yeast)
EUR 1015

Recombinant Human Glycogenin-1 (GYG1)

MBS966826-01mgEColi MyBiosource 0.1mg(E-Coli)
EUR 1060

Recombinant Human Glycogenin-1 (GYG1)

MBS966826-01mgYeast MyBiosource 0.1mg(Yeast)
EUR 1155

GYG1 (Glycogenin 1, GYG) (MaxLight 650)

MBS6227883-01mL MyBiosource 0.1(mL
EUR 875

GYG1 (Glycogenin 1, GYG) (MaxLight 650)

MBS6227883-5x01mL MyBiosource 5x0.1mL
EUR 3800

GYG1 (Glycogenin 1, GYG) (MaxLight 550)

MBS6217208-01mL MyBiosource 0.1(mL
EUR 875

GYG1 (Glycogenin 1, GYG) (MaxLight 550)

MBS6217208-5x01mL MyBiosource 5x0.1mL
EUR 3800

GYG1 (Glycogenin 1, GYG) (MaxLight 405)

MBS6195858-01mL MyBiosource 0.1(mL
EUR 875

GYG1 (Glycogenin 1, GYG) (MaxLight 405)

MBS6195858-5x01mL MyBiosource 5x0.1mL
EUR 3800

GYG1 (Glycogenin 1, GYG) (MaxLight 490)

MBS6206533-01mL MyBiosource 0.1(mL
EUR 875

GYG1 (Glycogenin 1, GYG) (MaxLight 490)

MBS6206533-5x01mL MyBiosource 5x0.1mL
EUR 3800

GYG1 (Glycogenin 1, GYG) (MaxLight 750)

MBS6238558-01mL MyBiosource 0.1(mL
EUR 875

GYG1 (Glycogenin 1, GYG) (MaxLight 750)

MBS6238558-5x01mL MyBiosource 5x0.1mL
EUR 3800

GYG1 (untagged)-Human glycogenin 1 (GYG1), transcript variant 1

SC117541 Origene Technologies GmbH 10 µg Ask for price

GYG1 (untagged)-Human glycogenin 1 (GYG1) transcript variant 3

SC328457 Origene Technologies GmbH 10 µg Ask for price

GYG1 (untagged)-Human glycogenin 1 (GYG1) transcript variant 2

SC328539 Origene Technologies GmbH 10 µg Ask for price

GYG1 (GFP-tagged) - Human glycogenin 1 (GYG1), transcript variant 1

RG218638 Origene Technologies GmbH 10 µg Ask for price

Gyg1 (Myc-DDK-tagged ORF) - Rat glycogenin 1 (Gyg1), (10 ug)

RR208175 Origene Technologies GmbH 10 µg Ask for price

Glycogenin 1 (GYG1) (NM_001184721) Human Recombinant Protein

E45H31383E1 EnoGene 50 ug
EUR 552.5

GYG1 (GFP-tagged) - Human glycogenin 1 (GYG1), transcript variant 3

RG229819 Origene Technologies GmbH 10 µg Ask for price

GYG1 (GFP-tagged) - Human glycogenin 1 (GYG1), transcript variant 2

RG229901 Origene Technologies GmbH 10 µg Ask for price

GYG1 (Myc-DDK-tagged)-Human glycogenin 1 (GYG1), transcript variant 1

RC218638 Origene Technologies GmbH 10 µg Ask for price

Glycogenin, Recombinant Human (GYG1, GYG)

MBS637445-01mg MyBiosource 0.1mg
EUR 540

Glycogenin, Recombinant Human (GYG1, GYG)

MBS637445-5x01mg MyBiosource 5x0.1mg
EUR 2275

Glycogenin 1 (GYG1) (NM_001184720) Human Over-expression Lysate

E45H00523-2 EnoGene each
EUR 395

Glycogenin 1 (GYG1) (NM_001184720) Human Over-expression Lysate

LS002992 BosterBio 100ug
EUR 628
Description: Transient overexpression lysate of glycogenin 1 (GYG1)

GYG1 (Myc-DDK-tagged)-Human glycogenin 1 (GYG1), transcript variant 2

RC229901 Origene Technologies GmbH 10 µg Ask for price

GYG1 (Myc-DDK-tagged)-Human glycogenin 1 (GYG1), transcript variant 3

RC229819 Origene Technologies GmbH 10 µg Ask for price

Lenti ORF clone of Gyg1 (mGFP-tagged ORF) - Rat glycogenin 1 (Gyg1), (10 ug)

RR208175L4 Origene Technologies GmbH 10 µg Ask for price

Glycogenin 1 (GYG1) (1-74) mouse monoclonal antibody, clone 3B5

AM20892PU-N Origene Technologies GmbH 50 µg Ask for price

Human Glycogenin 2 (GYG2)ELISA Kit

201-12-2720 Shanghai Sunred Biological Technology 96 tests
EUR 528
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human Glycogenin 2 (GYG2) ELISA Kit

abx387725-96tests Abbexa 96 tests
EUR 687.5

Human Glycogenin 2,GYG2 ELISA KIT

E2719Hu-1096T Jiaxing Korain Biotech Ltd (BT Labs) 10*96T
EUR 4122

Human Glycogenin 2,GYG2 ELISA KIT

E2719Hu-48wells Jiaxing Korain Biotech Ltd (BT Labs) 48 wells
EUR 300

Human Glycogenin 2,GYG2 ELISA KIT

E2719Hu-596T Jiaxing Korain Biotech Ltd (BT Labs) 5*96T
EUR 2061

Human Glycogenin 2,GYG2 ELISA KIT

E2719Hu-96wells Jiaxing Korain Biotech Ltd (BT Labs) 96 wells
EUR 458

Human Glycogenin 2,GYG2 ELISA KIT

JOT-EK2719Hu Jotbody 96 wells Ask for price
Description: Human

Human Glycogenin- 2, GYG2 ELISA KIT

ELI-09732h Lifescience Market 96 Tests
EUR 988.8

Human Glycogenin 2 (GYG2) ELISA Kit

MBS163588-10x96StripWells MyBiosource 10x96-Strip-Wells
EUR 3460

Human Glycogenin 2 (GYG2) ELISA Kit

MBS163588-48StripWells MyBiosource 48-Strip-Wells
EUR 285

Human Glycogenin 2 (GYG2) ELISA Kit

MBS163588-5x96StripWells MyBiosource 5x96-Strip-Wells
EUR 1750

Human Glycogenin 2 (GYG2) ELISA Kit

MBS163588-96StripWells MyBiosource 96-Strip-Wells
EUR 425

Human Glycogenin 2 (GYG2) ELISA Kit

MBS9303081-10x96StripWells MyBiosource 10x96-Strip-Wells
EUR 6725

Human Glycogenin 2 (GYG2) ELISA Kit

MBS9303081-48StripWells MyBiosource 48-Strip-Wells
EUR 550

Human Glycogenin 2 (GYG2) ELISA Kit

MBS9303081-5x96StripWells MyBiosource 5x96-Strip-Wells
EUR 3420

Human Glycogenin 2 (GYG2) ELISA Kit

MBS9303081-96StripWells MyBiosource 96-Strip-Wells
EUR 765

Human Glycogenin 2 (GYG2)ELISA Kit

YLA1959HU-48T Shanghai YL Biotech 48T Ask for price

Human Glycogenin 2 (GYG2)ELISA Kit

YLA1959HU-96T Shanghai YL Biotech 96T Ask for price

Lenti ORF clone of Gyg1 (Myc-DDK-tagged ORF) - Rat glycogenin 1 (Gyg1), (10 ug)

RR208175L3 Origene Technologies GmbH 10 µg Ask for price

Lenti-ORF clone of GYG1 (mGFP-tagged)-Human glycogenin 1 (GYG1), transcript variant 1

RC218638L4 Origene Technologies GmbH 10 µg Ask for price

Human Glycogenin-2 (GYG2) ELISA Kit

MBS280376-10x96StripWells MyBiosource 10x96-Strip-Wells
EUR 6255

Human Glycogenin-2 (GYG2) ELISA Kit

MBS280376-48StripWells MyBiosource 48-Strip-Wells
EUR 445

Human Glycogenin-2 (GYG2) ELISA Kit

MBS280376-5x96StripWells MyBiosource 5x96-Strip-Wells
EUR 3290

Human Glycogenin-2 (GYG2) ELISA Kit

MBS280376-96StripWells MyBiosource 96-Strip-Wells
EUR 730

Lenti ORF particles, Gyg1 (GFP-tagged ORF) - Rat glycogenin 1 (Gyg1), 200ul, >10^7 TU/mL

RR208175L4V Origene Technologies GmbH 200 µl Ask for price

Lenti-ORF clone of GYG1 (Myc-DDK-tagged)-Human glycogenin 1 (GYG1), transcript variant 1

RC218638L3 Origene Technologies GmbH 10 µg Ask for price

Lenti ORF particles, Gyg1 (Myc-DDK-tagged ORF) - Rat glycogenin 1 (Gyg1), 200ul, >10^7 TU/mL

RR208175L3V Origene Technologies GmbH 200 µl Ask for price

3`UTR clone of glycogenin 1 (GYG1) for miRNA target validation

SC209071 Origene Technologies GmbH 10 µg Ask for price

Rabbit polyclonal antibody to GYG2 (glycogenin 2)

TA308221 Origene Technologies GmbH 100 µl Ask for price

Lenti ORF particles, GYG1 (mGFP-tagged)-Human glycogenin 1 (GYG1), transcript variant 1, 200ul, >10^7 TU/mL

RC218638L4V Origene Technologies GmbH 200 µl Ask for price

Monoclonal GYG1 / Glycogenin Antibody (clone 3B5), Clone: 3B5

AMM05275G Leading Biology 0.05mg
EUR 633.6
Description: A Monoclonal antibody against Human GYG1 / Glycogenin (clone 3B5). The antibodies are raised in Mouse and are from clone 3B5. This antibody is applicable in WB and IHC-P, E

Lenti ORF particles, GYG1 (mGFP-tagged) - Human glycogenin 1 (GYG1), transcript variant 2, 200ul, >10^7 TU/mL

RC229901L4V Origene Technologies GmbH 200 µl Ask for price

Lenti ORF particles, GYG1 (mGFP-tagged) - Human glycogenin 1 (GYG1), transcript variant 3, 200ul, >10^7 TU/mL

RC229819L4V Origene Technologies GmbH 200 µl Ask for price

Lenti ORF particles, GYG1 (Myc-DDK-tagged)-Human glycogenin 1 (GYG1), transcript variant 1, 200ul, >10^7 TU/mL

RC218638L3V Origene Technologies GmbH 200 µl Ask for price

Human Glycogenin 2, GYG2 GENLISA ELISA

KBH2719 Krishgen 1 x 96 wells
EUR 286

Lenti ORF particles, GYG1 (Myc-DDK tagged) - Human glycogenin 1 (GYG1), transcript variant 2, 200ul, >10^7 TU/mL

RC229901L3V Origene Technologies GmbH 200 µl Ask for price

Lenti ORF particles, GYG1 (Myc-DDK tagged) - Human glycogenin 1 (GYG1), transcript variant 3, 200ul, >10^7 TU/mL

RC229819L3V Origene Technologies GmbH 200 µl Ask for price

GYG2 (Glycogenin 2, Glycogenin-2, GN-2, GN2) (AP)

MBS6380660-01mL MyBiosource 0.1mL
EUR 920

GYG2 (Glycogenin 2, Glycogenin-2, GN-2, GN2) (AP)

MBS6380660-5x01mL MyBiosource 5x0.1mL
EUR 3990

GYG2 (Glycogenin 2, Glycogenin-2, GN-2, GN2) (PE)

MBS6380670-01mL MyBiosource 0.1mL
EUR 920

GYG2 (Glycogenin 2, Glycogenin-2, GN-2, GN2) (PE)

MBS6380670-5x01mL MyBiosource 5x0.1mL
EUR 3990

GYG2 (Glycogenin 2, Glycogenin-2, GN-2, GN2) (PE)

MBS6158121-01mL MyBiosource 0.1(mL
EUR 875

GYG2 (Glycogenin 2, Glycogenin-2, GN-2, GN2) (PE)

MBS6158121-5x01mL MyBiosource 5x0.1mL
EUR 3800

GYG2 (Glycogenin 2, Glycogenin-2, GN-2, GN2) (AP)

MBS6131606-01mL MyBiosource 0.1(mL
EUR 875

GYG2 (Glycogenin 2, Glycogenin-2, GN-2, GN2) (AP)

MBS6131606-5x01mL MyBiosource 5x0.1mL
EUR 3800

GYG2 (Glycogenin 2, Glycogenin-2, GN-2, GN2) APC

MBS6380661-01mL MyBiosource 0.1mL
EUR 920

GYG2 (Glycogenin 2, Glycogenin-2, GN-2, GN2) APC

MBS6380661-5x01mL MyBiosource 5x0.1mL
EUR 3990

GYG2 (Glycogenin 2, Glycogenin-2, GN-2, GN2) (HRP)

MBS6380664-01mL MyBiosource 0.1mL
EUR 920

GYG2 (Glycogenin 2, Glycogenin-2, GN-2, GN2) (HRP)

MBS6380664-5x01mL MyBiosource 5x0.1mL
EUR 3990

GYG2 (Glycogenin 2, Glycogenin-2, GN-2, GN2) (HRP)

MBS6152818-01mL MyBiosource 0.1(mL
EUR 875

GYG2 (Glycogenin 2, Glycogenin-2, GN-2, GN2) (HRP)

MBS6152818-5x01mL MyBiosource 5x0.1mL
EUR 3800
×

Most subfamilies seem monofunctional (i.e. include enzymes with the similar substrate and the similar product). The shut examination of the different, apparently polyspecific, subfamilies revealed that they really group collectively enzymes with strongly associated (and even generally nearly equivalent) actions. Overall our subfamily task permits to set the limits for genomic perform prediction on this huge household of biologically and industrially vital enzymes.

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Recent Posts

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  • NCBI References of Lab Assays
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Recent Posts

  • Manufacturers of Lab Assays
  • NCBI References of Lab Assays
  • Antibody
  • cDNA
  • DNA
  • Elisa Kits
  • Hamster
  • HEPES
  • Heterologous expression and mutagenesis of recombinant Vespa affinis hyaluronidase protein (rVesA2).
  • high
  • Horse
  • Horse serum
  • host
  • HRP
  • In Planta Glycan Engineering and Functional Activities of IgE Antibodies.
  • phosphor
  • reverse
  • RIA
  • rnai
  • TEMED
  • The mucin-selective protease StcE enables molecular and functional analysis of human cancer-associated mucins.
  • Tomato UDP-Glucose Sterol Glycosyltransferases: A Family of Developmental and Stress Regulated Genes that Encode Cytosolic and Membrane-Associated Forms of the Enzyme.
  • Two Novel Fungal Phenolic UDP Glycosyltransferases from Absidia coerulea and Rhizopus japonicus.
  • UGT85A84 Catalyzes the Glycosylation of Aromatic Monoterpenes in Osmanthus fragrans Lour. Flowers.
  • Manufacturers of Lab Assays
  • NCBI References of Lab Assays
  • VWR alternatives of Lab recombinants
  • References of Lab rec.
  • Compare ELISA lab reagents for research
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