Stimulation of lignocellulosic biomass hydrolysis by proteins of glycoside hydrolase family 61: structure and function of a large, enigmatic family.

Stimulation of lignocellulosic biomass hydrolysis by proteins of glycoside hydrolase family 61: structure and function of a large, enigmatic family.

Posted on by Mathew

Currently, the comparatively excessive price of enzymes resembling glycoside hydrolases that catalyze cellulose hydrolysis represents a barrier to commercialization of a biorefinery succesful of producing renewable transportable fuels resembling ethanol from ample lignocellulosic biomass. Among the numerous households of glycoside hydrolases that catalyze cellulose and hemicellulose hydrolysis, few are extra enigmatic than family 61 (GH61), initially categorized primarily based on measurement of very weak endo-1,4-beta-d-glucanase exercise in a single family member.

Here we present that sure GH61 proteins lack measurable hydrolytic exercise by themselves however within the presence of varied divalent metallic ions can considerably scale back the overall protein loading required to hydrolyze lignocellulosic biomass. We additionally solved the structure of one extremely lively GH61 protein and discover that it’s devoid of conserved, carefully juxtaposed acidic aspect chains that would function normal proton donor and nucleophile/base in a canonical hydrolytic response, and we conclude that the GH61 proteins are unlikely to be glycoside hydrolases.

Structure-based mutagenesis exhibits the significance of a number of conserved residues for GH61 function. By incorporating the gene for one GH61 protein into a industrial Trichoderma reesei pressure producing excessive ranges of cellulolytic enzymes, we’re in a position to scale back by 2-fold the overall protein loading (and therefore the associated fee) required to hydrolyze lignocellulosic biomass. Several members of the glycoside hydrolase 61 (GH61) family of proteins have lately been proven to dramatically improve the breakdown of lignocellulosic biomass by microbial hydrolytic cellulases.

However, purified GH61 proteins have neither demonstrable direct hydrolase exercise on varied polysaccharide or lignacious parts of biomass nor an obvious hydrolase lively web site. Cellobiose dehydrogenase (CDH) is a secreted flavocytochrome produced by many cellulose-degrading fungi with no well-understood organic function. Here we exhibit that the binary mixture of Thermoascus aurantiacus GH61A (TaGH61A) and Humicola insolens CDH (HiCDH) cleaves cellulose into soluble, oxidized oligosaccharides.

Cardiac glycosides as novel most cancers therapeutic brokers.

The class of steroid-like compounds designated cardiac glycosides contains well-known medicine resembling digoxin, digitoxin, and ouabain. Their continued efficacy in therapy of congestive coronary heart failure and as anti-arrhythmic brokers is properly appreciated. Less well-known, nonetheless, is the rising function of this class of compounds within the prevention and/or therapy of proliferative illnesses resembling most cancers.

New findings throughout the previous 5 years have revealed these compounds to be concerned in complicated cell-signal transduction mechanisms, leading to selective management of human tumor however not regular mobile proliferation. As such, they characterize a promising kind of focused most cancers chemotherapy. New scientific research of their anticancer potential as single or adjuvant therapies might present perception into these probably useful therapeutic choices.

Stimulation of lignocellulosic biomass hydrolysis by proteins of glycoside hydrolase family 61: structure and function of a large, enigmatic family.

This evaluate focuses on latest findings on mobile pharmacology of cardiac glycosides as they relate to therapy of human most cancers and makes an attempt to elucidate why these brokers have been missed previously. TaGH61A-HiCDH exercise on cellulose is proven to be nonredundant with the actions of canonical endocellulase and exocellulase enzymes in microcrystalline cellulose cleavage, and whereas the mixture of TaGH61A and HiCDH cleaves extremely crystalline bacterial cellulose, it doesn’t cleave soluble cellodextrins.

GH61 and CDH proteins are coexpressed and secreted by the thermophilic ascomycete Thielavia terrestris in response to environmental cellulose, and the mixed actions of T. terrestris GH61 and T. terrestris CDH are proven to synergize with T. terrestris cellulose hydrolases within the breakdown of cellulose. The motion of GH61 and CDH on cellulose might represent an essential, however missed, organic oxidoreductive system that features in microbial lignocellulose degradation and has purposes in industrial biomass utilization.

Cardiac glycosides exert anticancer results by inducing immunogenic cell demise.

Some profitable chemotherapeutics, notably anthracyclines and oxaliplatin, induce a kind of cell stress and demise that’s immunogenic, therefore changing the affected person’s dying most cancers cells into a vaccine that stimulates antitumor immune responses.

By means of a fluorescence microscopy platform that permits for the automated detection of the biochemical hallmarks of such a peculiar cell demise modality, we recognized cardiac glycosides (CGs) as exceptionally environment friendly inducers of immunogenic cell demise, an impact that was related to the inhibition of the plasma membrane Na(+)- and Okay(+)-dependent adenosine triphosphatase (Na(+)/Okay(+)-ATPase). CGs exacerbated the antineoplastic results of DNA-damaging brokers in immunocompetent however not immunodeficient mice.

Moreover, most cancers cells succumbing to a mixture of chemotherapy plus CGs may vaccinate syngeneic mice in opposition to a subsequent problem with residing cells of the identical kind. Finally, retrospective scientific analyses revealed that the administration of the CG digoxin throughout chemotherapy had a optimistic affect on total survival in cohorts of breast, colorectal, head and neck, and hepatocellular carcinoma sufferers, particularly once they had been handled with brokers apart from anthracyclines and oxaliplatin.

Metagenomic and bioinformatic approaches had been used to characterize plant biomass conversion throughout the foregut microbiome of Australia’s “mannequin” marsupial, the Tammar wallaby (Macropus eugenii). Like the termite hindgut and bovine rumen, key enzymes and modular buildings attribute of the “free enzyme” and “cellulosome” paradigms of cellulose solubilization stay both poorly represented or elusive to seize by shotgun sequencing strategies.

Instead, multigene polysaccharide utilization loci-like programs coupled with genes encoding beta-1,4-endoglucanases and beta-1,4-endoxylanases–which haven’t been beforehand encountered in metagenomic datasets–were recognized, as had been a numerous set of glycoside hydrolases focusing on noncellulosic polysaccharides.

Recombinant Rhodopirellula baltica Putative 3-methyladenine DNA glycosylase (mpg)
MBS1422697-002mgYeast 0.02mg(Yeast)
EUR 905

Recombinant Rhodopirellula baltica Putative 3-methyladenine DNA glycosylase (mpg)
MBS1422697-01mgEColi 0.1mg(E-Coli)
EUR 890

Recombinant Rhodopirellula baltica Putative 3-methyladenine DNA glycosylase (mpg)
MBS1422697-01mgYeast 0.1mg(Yeast)
EUR 1060

MPG (DNA-3-methyladenine Glycosylase, 3-alkyladenine DNA Glycosylase, 3-methyladenine DNA Glycosidase, ADPG, N-methylpurine-DNA Glycosylase, AAG, ANPG, MID1) (AP)
MBS6132360-01mL 0.1(mL
EUR 875

MPG (DNA-3-methyladenine Glycosylase, 3-alkyladenine DNA Glycosylase, 3-methyladenine DNA Glycosidase, ADPG, N-methylpurine-DNA Glycosylase, AAG, ANPG, MID1) (AP)
MBS6132360-5x01mL 5x0.1mL
EUR 3800

MPG (DNA-3-methyladenine Glycosylase, 3-alkyladenine DNA Glycosylase, 3-methyladenine DNA Glycosidase, ADPG, N-methylpurine-DNA Glycosylase, AAG, ANPG, MID1) APC
MBS6137663-01mL 0.1(mL
EUR 875

MPG (DNA-3-methyladenine Glycosylase, 3-alkyladenine DNA Glycosylase, 3-methyladenine DNA Glycosidase, ADPG, N-methylpurine-DNA Glycosylase, AAG, ANPG, MID1) APC
MBS6137663-5x01mL 5x0.1mL
EUR 3800

MPG (DNA-3-methyladenine Glycosylase, 3-alkyladenine DNA Glycosylase, 3-methyladenine DNA Glycosidase, ADPG, N-methylpurine-DNA Glycosylase, AAG, ANPG, MID1) (Biotin)
MBS6142966-01mL 0.1(mL
EUR 875

MPG (DNA-3-methyladenine Glycosylase, 3-alkyladenine DNA Glycosylase, 3-methyladenine DNA Glycosidase, ADPG, N-methylpurine-DNA Glycosylase, AAG, ANPG, MID1) (Biotin)
MBS6142966-5x01mL 5x0.1mL
EUR 3800

MPG (DNA-3-methyladenine Glycosylase, 3-alkyladenine DNA Glycosylase, 3-methyladenine DNA Glycosidase, ADPG, N-methylpurine-DNA Glycosylase, AAG, ANPG, MID1) (FITC)
MBS6148269-01mL 0.1(mL
EUR 875

MPG (DNA-3-methyladenine Glycosylase, 3-alkyladenine DNA Glycosylase, 3-methyladenine DNA Glycosidase, ADPG, N-methylpurine-DNA Glycosylase, AAG, ANPG, MID1) (FITC)
MBS6148269-5x01mL 5x0.1mL
EUR 3800

MPG (DNA-3-methyladenine Glycosylase, 3-alkyladenine DNA Glycosylase, 3-methyladenine DNA Glycosidase, ADPG, N-methylpurine-DNA Glycosylase, AAG, ANPG, MID1) (AP)
MBS6385775-01mL 0.1mL
EUR 920

MPG (DNA-3-methyladenine Glycosylase, 3-alkyladenine DNA Glycosylase, 3-methyladenine DNA Glycosidase, ADPG, N-methylpurine-DNA Glycosylase, AAG, ANPG, MID1) (AP)
MBS6385775-5x01mL 5x0.1mL
EUR 3990

MPG (DNA-3-methyladenine Glycosylase, 3-alkyladenine DNA Glycosylase, 3-methyladenine DNA Glycosidase, ADPG, N-methylpurine-DNA Glycosylase, AAG, ANPG, MID1) APC
MBS6385776-01mL 0.1mL
EUR 920

MPG (DNA-3-methyladenine Glycosylase, 3-alkyladenine DNA Glycosylase, 3-methyladenine DNA Glycosidase, ADPG, N-methylpurine-DNA Glycosylase, AAG, ANPG, MID1) APC
MBS6385776-5x01mL 5x0.1mL
EUR 3990

MPG (DNA-3-methyladenine Glycosylase, 3-alkyladenine DNA Glycosylase, 3-methyladenine DNA Glycosidase, ADPG, N-methylpurine-DNA Glycosylase, AAG, ANPG, MID1) (Biotin)
MBS6385777-01mL 0.1mL
EUR 920

MPG (DNA-3-methyladenine Glycosylase, 3-alkyladenine DNA Glycosylase, 3-methyladenine DNA Glycosidase, ADPG, N-methylpurine-DNA Glycosylase, AAG, ANPG, MID1) (Biotin)
MBS6385777-5x01mL 5x0.1mL
EUR 3990

MPG (DNA-3-methyladenine Glycosylase, 3-alkyladenine DNA Glycosylase, 3-methyladenine DNA Glycosidase, ADPG, N-methylpurine-DNA Glycosylase, AAG, ANPG, MID1) (FITC)
MBS6385778-01mL 0.1mL
EUR 920

MPG (DNA-3-methyladenine Glycosylase, 3-alkyladenine DNA Glycosylase, 3-methyladenine DNA Glycosidase, ADPG, N-methylpurine-DNA Glycosylase, AAG, ANPG, MID1) (FITC)
MBS6385778-5x01mL 5x0.1mL
EUR 3990

MPG (DNA-3-methyladenine Glycosylase, 3-alkyladenine DNA Glycosylase, 3-methyladenine DNA Glycosidase, ADPG, N-methylpurine-DNA Glycosylase, AAG, ANPG, MID1) (HRP)
MBS6385779-01mL 0.1mL
EUR 920

MPG (DNA-3-methyladenine Glycosylase, 3-alkyladenine DNA Glycosylase, 3-methyladenine DNA Glycosidase, ADPG, N-methylpurine-DNA Glycosylase, AAG, ANPG, MID1) (HRP)
MBS6385779-5x01mL 5x0.1mL
EUR 3990

MPG (DNA-3-methyladenine Glycosylase, 3-alkyladenine DNA Glycosylase, 3-methyladenine DNA Glycosidase, ADPG, N-methylpurine-DNA Glycosylase, AAG, ANPG, MID1) (PE)
MBS6385785-01mL 0.1mL
EUR 920

MPG (DNA-3-methyladenine Glycosylase, 3-alkyladenine DNA Glycosylase, 3-methyladenine DNA Glycosidase, ADPG, N-methylpurine-DNA Glycosylase, AAG, ANPG, MID1) (PE)
MBS6385785-5x01mL 5x0.1mL
EUR 3990

MPG (DNA-3-methyladenine Glycosylase, 3-alkyladenine DNA Glycosylase, 3-methyladenine DNA Glycosidase, ADPG, N-methylpurine-DNA Glycosylase, AAG, ANPG, MID1) (PE)
MBS6158875-01mL 0.1(mL
EUR 875

MPG (DNA-3-methyladenine Glycosylase, 3-alkyladenine DNA Glycosylase, 3-methyladenine DNA Glycosidase, ADPG, N-methylpurine-DNA Glycosylase, AAG, ANPG, MID1) (PE)
MBS6158875-5x01mL 5x0.1mL
EUR 3800

MPG (DNA-3-methyladenine Glycosylase, 3-alkyladenine DNA Glycosylase, 3-methyladenine DNA Glycosidase, ADPG, N-methylpurine-DNA Glycosylase, AAG, ANPG, MID1) (HRP)
MBS6153572-01mL 0.1(mL
EUR 875

MPG (DNA-3-methyladenine Glycosylase, 3-alkyladenine DNA Glycosylase, 3-methyladenine DNA Glycosidase, ADPG, N-methylpurine-DNA Glycosylase, AAG, ANPG, MID1) (HRP)
MBS6153572-5x01mL 5x0.1mL
EUR 3800

MPG (DNA-3-methyladenine Glycosylase, 3-alkyladenine DNA Glycosylase, 3-methyladenine DNA Glycosidase, ADPG, N-methylpurine-DNA Glycosylase, AAG, ANPG, MID1) (MaxLight 550)
MBS6212573-01mL 0.1(mL
EUR 875

MPG (DNA-3-methyladenine Glycosylase, 3-alkyladenine DNA Glycosylase, 3-methyladenine DNA Glycosidase, ADPG, N-methylpurine-DNA Glycosylase, AAG, ANPG, MID1) (MaxLight 550)
MBS6212573-5x01mL 5x0.1mL
EUR 3800

MPG (DNA-3-methyladenine Glycosylase, 3-alkyladenine DNA Glycosylase, 3-methyladenine DNA Glycosidase, ADPG, N-methylpurine-DNA Glycosylase, AAG, ANPG, MID1) (MaxLight 405)
MBS6191223-01mL 0.1(mL
EUR 875

MPG (DNA-3-methyladenine Glycosylase, 3-alkyladenine DNA Glycosylase, 3-methyladenine DNA Glycosidase, ADPG, N-methylpurine-DNA Glycosylase, AAG, ANPG, MID1) (MaxLight 405)
MBS6191223-5x01mL 5x0.1mL
EUR 3800

MPG (DNA-3-methyladenine Glycosylase, 3-alkyladenine DNA Glycosylase, 3-methyladenine DNA Glycosidase, ADPG, N-methylpurine-DNA Glycosylase, AAG, ANPG, MID1) (MaxLight 750)
MBS6233923-01mL 0.1(mL
EUR 875

MPG (DNA-3-methyladenine Glycosylase, 3-alkyladenine DNA Glycosylase, 3-methyladenine DNA Glycosidase, ADPG, N-methylpurine-DNA Glycosylase, AAG, ANPG, MID1) (MaxLight 750)
MBS6233923-5x01mL 5x0.1mL
EUR 3800

MPG (DNA-3-methyladenine Glycosylase, 3-alkyladenine DNA Glycosylase, 3-methyladenine DNA Glycosidase, ADPG, N-methylpurine-DNA Glycosylase, AAG, ANPG, MID1) (MaxLight 490)
MBS6201898-01mL 0.1(mL
EUR 875

MPG (DNA-3-methyladenine Glycosylase, 3-alkyladenine DNA Glycosylase, 3-methyladenine DNA Glycosidase, ADPG, N-methylpurine-DNA Glycosylase, AAG, ANPG, MID1) (MaxLight 490)
MBS6201898-5x01mL 5x0.1mL
EUR 3800

MPG (DNA-3-methyladenine Glycosylase, 3-alkyladenine DNA Glycosylase, 3-methyladenine DNA Glycosidase, ADPG, N-methylpurine-DNA Glycosylase, AAG, ANPG, MID1) (MaxLight 405)
MBS6385780-01mL 0.1mL
EUR 920

MPG (DNA-3-methyladenine Glycosylase, 3-alkyladenine DNA Glycosylase, 3-methyladenine DNA Glycosidase, ADPG, N-methylpurine-DNA Glycosylase, AAG, ANPG, MID1) (MaxLight 405)
MBS6385780-5x01mL 5x0.1mL
EUR 3990

MPG (DNA-3-methyladenine Glycosylase, 3-alkyladenine DNA Glycosylase, 3-methyladenine DNA Glycosidase, ADPG, N-methylpurine-DNA Glycosylase, AAG, ANPG, MID1) (MaxLight 490)
MBS6385781-01mL 0.1mL
EUR 920

MPG (DNA-3-methyladenine Glycosylase, 3-alkyladenine DNA Glycosylase, 3-methyladenine DNA Glycosidase, ADPG, N-methylpurine-DNA Glycosylase, AAG, ANPG, MID1) (MaxLight 490)
MBS6385781-5x01mL 5x0.1mL
EUR 3990

MPG (DNA-3-methyladenine Glycosylase, 3-alkyladenine DNA Glycosylase, 3-methyladenine DNA Glycosidase, ADPG, N-methylpurine-DNA Glycosylase, AAG, ANPG, MID1) (MaxLight 550)
MBS6385782-01mL 0.1mL
EUR 920

MPG (DNA-3-methyladenine Glycosylase, 3-alkyladenine DNA Glycosylase, 3-methyladenine DNA Glycosidase, ADPG, N-methylpurine-DNA Glycosylase, AAG, ANPG, MID1) (MaxLight 550)
MBS6385782-5x01mL 5x0.1mL
EUR 3990

MPG (DNA-3-methyladenine Glycosylase, 3-alkyladenine DNA Glycosylase, 3-methyladenine DNA Glycosidase, ADPG, N-methylpurine-DNA Glycosylase, AAG, ANPG, MID1) (MaxLight 650)
MBS6385783-01mL 0.1mL
EUR 920

MPG (DNA-3-methyladenine Glycosylase, 3-alkyladenine DNA Glycosylase, 3-methyladenine DNA Glycosidase, ADPG, N-methylpurine-DNA Glycosylase, AAG, ANPG, MID1) (MaxLight 650)
MBS6385783-5x01mL 5x0.1mL
EUR 3990

MPG (DNA-3-methyladenine Glycosylase, 3-alkyladenine DNA Glycosylase, 3-methyladenine DNA Glycosidase, ADPG, N-methylpurine-DNA Glycosylase, AAG, ANPG, MID1) (MaxLight 750)
MBS6385784-01mL 0.1mL
EUR 920

MPG (DNA-3-methyladenine Glycosylase, 3-alkyladenine DNA Glycosylase, 3-methyladenine DNA Glycosidase, ADPG, N-methylpurine-DNA Glycosylase, AAG, ANPG, MID1) (MaxLight 750)
MBS6385784-5x01mL 5x0.1mL
EUR 3990

MPG (DNA-3-methyladenine Glycosylase, 3-alkyladenine DNA Glycosylase, 3-methyladenine DNA Glycosidase, ADPG, N-methylpurine-DNA Glycosylase, AAG, ANPG, MID1) (MaxLight 650)
MBS6223248-01mL 0.1(mL
EUR 875

MPG (DNA-3-methyladenine Glycosylase, 3-alkyladenine DNA Glycosylase, 3-methyladenine DNA Glycosidase, ADPG, N-methylpurine-DNA Glycosylase, AAG, ANPG, MID1) (MaxLight 650)
MBS6223248-5x01mL 5x0.1mL
EUR 3800

Recombinant Bacillus cereus Putative 3-methyladenine DNA glycosylase (BC_0885)
MBS1287994-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1125

Recombinant Bacillus cereus Putative 3-methyladenine DNA glycosylase (BC_0885)
MBS1287994-002mgEColi 0.02mg(E-Coli)
EUR 705

Recombinant Bacillus cereus Putative 3-methyladenine DNA glycosylase (BC_0885)
MBS1287994-002mgYeast 0.02mg(Yeast)
EUR 880

Recombinant Bacillus cereus Putative 3-methyladenine DNA glycosylase (BC_0885)
MBS1287994-01mgEColi 0.1mg(E-Coli)
EUR 850

Recombinant Bacillus cereus Putative 3-methyladenine DNA glycosylase (BC_0885)
MBS1287994-01mgYeast 0.1mg(Yeast)
EUR 1025

Recombinant Rickettsia felis Putative 3-methyladenine DNA glycosylase (RF_0560)
MBS1311410-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1100

Recombinant Rickettsia felis Putative 3-methyladenine DNA glycosylase (RF_0560)
MBS1311410-002mgEColi 0.02mg(E-Coli)
EUR 695

Recombinant Rickettsia felis Putative 3-methyladenine DNA glycosylase (RF_0560)
MBS1311410-002mgYeast 0.02mg(Yeast)
EUR 850

Recombinant Rickettsia felis Putative 3-methyladenine DNA glycosylase (RF_0560)
MBS1311410-01mgEColi 0.1mg(E-Coli)
EUR 805

Recombinant Rickettsia felis Putative 3-methyladenine DNA glycosylase (RF_0560)
MBS1311410-01mgYeast 0.1mg(Yeast)
EUR 995

Recombinant Borrelia burgdorferi Putative 3-methyladenine DNA glycosylase (BB_0422)
MBS1147654-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1105

Recombinant Borrelia burgdorferi Putative 3-methyladenine DNA glycosylase (BB_0422)
MBS1147654-002mgEColi 0.02mg(E-Coli)
EUR 700

Recombinant Borrelia burgdorferi Putative 3-methyladenine DNA glycosylase (BB_0422)
MBS1147654-002mgYeast 0.02mg(Yeast)
EUR 860

Recombinant Borrelia burgdorferi Putative 3-methyladenine DNA glycosylase (BB_0422)
MBS1147654-01mgEColi 0.1mg(E-Coli)
EUR 820

Recombinant Borrelia burgdorferi Putative 3-methyladenine DNA glycosylase (BB_0422)
MBS1147654-01mgYeast 0.1mg(Yeast)
EUR 1005

Recombinant Enterococcus faecalis Putative 3-methyladenine DNA glycosylase (EF_1978)
MBS1456048-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1120

Recombinant Enterococcus faecalis Putative 3-methyladenine DNA glycosylase (EF_1978)
MBS1456048-002mgEColi 0.02mg(E-Coli)
EUR 735

Recombinant Enterococcus faecalis Putative 3-methyladenine DNA glycosylase (EF_1978)
MBS1456048-002mgYeast 0.02mg(Yeast)
EUR 900

Recombinant Enterococcus faecalis Putative 3-methyladenine DNA glycosylase (EF_1978)
MBS1456048-01mgEColi 0.1mg(E-Coli)
EUR 885

Recombinant Enterococcus faecalis Putative 3-methyladenine DNA glycosylase (EF_1978)
MBS1456048-01mgYeast 0.1mg(Yeast)
EUR 1055

Recombinant Lactobacillus johnsonii Putative 3-methyladenine DNA glycosylase (LJ_0088)
MBS1281814-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1125

Recombinant Lactobacillus johnsonii Putative 3-methyladenine DNA glycosylase (LJ_0088)
MBS1281814-002mgEColi 0.02mg(E-Coli)
EUR 710

Recombinant Lactobacillus johnsonii Putative 3-methyladenine DNA glycosylase (LJ_0088)
MBS1281814-002mgYeast 0.02mg(Yeast)
EUR 880

Recombinant Lactobacillus johnsonii Putative 3-methyladenine DNA glycosylase (LJ_0088)
MBS1281814-01mgEColi 0.1mg(E-Coli)
EUR 855

Recombinant Lactobacillus johnsonii Putative 3-methyladenine DNA glycosylase (LJ_0088)
MBS1281814-01mgYeast 0.1mg(Yeast)
EUR 1030

Recombinant Deinococcus radiodurans Putative 3-methyladenine DNA glycosylase (DR_2074)
MBS1308297-005mgBaculovirus 0.05mg(Baculovirus)
EUR 1035

Recombinant Deinococcus radiodurans Putative 3-methyladenine DNA glycosylase (DR_2074)
MBS1308297-005mgEColi 0.05mg(E-Coli)
EUR 705

Recombinant Deinococcus radiodurans Putative 3-methyladenine DNA glycosylase (DR_2074)
MBS1308297-005mgYeast 0.05mg(Yeast)
EUR 860

Recombinant Deinococcus radiodurans Putative 3-methyladenine DNA glycosylase (DR_2074)
MBS1308297-02mgEColi 0.2mg(E-Coli)
EUR 915

Recombinant Deinococcus radiodurans Putative 3-methyladenine DNA glycosylase (DR_2074)
MBS1308297-05mgEColi 0.5mg(E-Coli)
EUR 1010

Recombinant Lactobacillus plantarum Putative 3-methyladenine DNA glycosylase (lp_1991)
MBS1403955-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1125

Recombinant Lactobacillus plantarum Putative 3-methyladenine DNA glycosylase (lp_1991)
MBS1403955-002mgEColi 0.02mg(E-Coli)
EUR 715

Recombinant Lactobacillus plantarum Putative 3-methyladenine DNA glycosylase (lp_1991)
MBS1403955-002mgYeast 0.02mg(Yeast)
EUR 880

Recombinant Lactobacillus plantarum Putative 3-methyladenine DNA glycosylase (lp_1991)
MBS1403955-01mgEColi 0.1mg(E-Coli)
EUR 855

Recombinant Lactobacillus plantarum Putative 3-methyladenine DNA glycosylase (lp_1991)
MBS1403955-01mgYeast 0.1mg(Yeast)
EUR 1030

Recombinant Ehrlichia ruminantium Putative 3-methyladenine DNA glycosylase
MBS1475503-005mgBaculovirus 0.05mg(Baculovirus)
EUR 1030

Recombinant Ehrlichia ruminantium Putative 3-methyladenine DNA glycosylase
MBS1475503-005mgEColi 0.05mg(E-Coli)
EUR 700

Recombinant Ehrlichia ruminantium Putative 3-methyladenine DNA glycosylase
MBS1475503-005mgYeast 0.05mg(Yeast)
EUR 860

Recombinant Ehrlichia ruminantium Putative 3-methyladenine DNA glycosylase
MBS1475503-02mgEColi 0.2mg(E-Coli)
EUR 915

Recombinant Ehrlichia ruminantium Putative 3-methyladenine DNA glycosylase
MBS1475503-05mgEColi 0.5mg(E-Coli)
EUR 1005

Recombinant Synechococcus sp. Putative 3-methyladenine DNA glycosylase (CYA_0301)
MBS1359471-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1115

Recombinant Synechococcus sp. Putative 3-methyladenine DNA glycosylase (CYA_0301)
MBS1359471-002mgEColi 0.02mg(E-Coli)
EUR 695

Recombinant Synechococcus sp. Putative 3-methyladenine DNA glycosylase (CYA_0301)
MBS1359471-002mgYeast 0.02mg(Yeast)
EUR 870

Recombinant Synechococcus sp. Putative 3-methyladenine DNA glycosylase (CYA_0301)
MBS1359471-01mgEColi 0.1mg(E-Coli)
EUR 830

Recombinant Synechococcus sp. Putative 3-methyladenine DNA glycosylase (CYA_0301)
MBS1359471-01mgYeast 0.1mg(Yeast)
EUR 1015

Recombinant Ehrlichia canis Putative 3-methyladenine DNA glycosylase (Ecaj_0750)
MBS1360522-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1110

Recombinant Ehrlichia canis Putative 3-methyladenine DNA glycosylase (Ecaj_0750)
MBS1360522-002mgEColi 0.02mg(E-Coli)
EUR 705

Recombinant Ehrlichia canis Putative 3-methyladenine DNA glycosylase (Ecaj_0750)
MBS1360522-002mgYeast 0.02mg(Yeast)
EUR 860

Recombinant Ehrlichia canis Putative 3-methyladenine DNA glycosylase (Ecaj_0750)
MBS1360522-01mgEColi 0.1mg(E-Coli)
EUR 825

Recombinant Ehrlichia canis Putative 3-methyladenine DNA glycosylase (Ecaj_0750)
MBS1360522-01mgYeast 0.1mg(Yeast)
EUR 1010

Recombinant Synechococcus sp. Putative 3-methyladenine DNA glycosylase (CYB_2817)
MBS1309244-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1110

Recombinant Synechococcus sp. Putative 3-methyladenine DNA glycosylase (CYB_2817)
MBS1309244-002mgEColi 0.02mg(E-Coli)
EUR 690

Recombinant Synechococcus sp. Putative 3-methyladenine DNA glycosylase (CYB_2817)
MBS1309244-002mgYeast 0.02mg(Yeast)
EUR 865

Recombinant Synechococcus sp. Putative 3-methyladenine DNA glycosylase (CYB_2817)
MBS1309244-01mgEColi 0.1mg(E-Coli)
EUR 830

Recombinant Synechococcus sp. Putative 3-methyladenine DNA glycosylase (CYB_2817)
MBS1309244-01mgYeast 0.1mg(Yeast)
EUR 1015

Recombinant Bacillus cereus Putative 3-methyladenine DNA glycosylase (BCE_0959)
MBS1297675-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1125

Recombinant Bacillus cereus Putative 3-methyladenine DNA glycosylase (BCE_0959)
MBS1297675-002mgEColi 0.02mg(E-Coli)
EUR 705

Recombinant Bacillus cereus Putative 3-methyladenine DNA glycosylase (BCE_0959)
MBS1297675-002mgYeast 0.02mg(Yeast)
EUR 880

Recombinant Bacillus cereus Putative 3-methyladenine DNA glycosylase (BCE_0959)
MBS1297675-01mgEColi 0.1mg(E-Coli)
EUR 850

Recombinant Bacillus cereus Putative 3-methyladenine DNA glycosylase (BCE_0959)
MBS1297675-01mgYeast 0.1mg(Yeast)
EUR 1025

Recombinant Frankia sp. Putative 3-methyladenine DNA glycosylase (Francci3_3167)
MBS1390619-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1130

Recombinant Frankia sp. Putative 3-methyladenine DNA glycosylase (Francci3_3167)
MBS1390619-002mgEColi 0.02mg(E-Coli)
EUR 715

Recombinant Frankia sp. Putative 3-methyladenine DNA glycosylase (Francci3_3167)
MBS1390619-002mgYeast 0.02mg(Yeast)
EUR 885

Recombinant Frankia sp. Putative 3-methyladenine DNA glycosylase (Francci3_3167)
MBS1390619-01mgEColi 0.1mg(E-Coli)
EUR 860

Recombinant Frankia sp. Putative 3-methyladenine DNA glycosylase (Francci3_3167)
MBS1390619-01mgYeast 0.1mg(Yeast)
EUR 1035

Recombinant Bacillus cereus Putative 3-methyladenine DNA glycosylase (BCQ_0959)
MBS1071677-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1125

Recombinant Bacillus cereus Putative 3-methyladenine DNA glycosylase (BCQ_0959)
MBS1071677-002mgEColi 0.02mg(E-Coli)
EUR 705

Recombinant Bacillus cereus Putative 3-methyladenine DNA glycosylase (BCQ_0959)
MBS1071677-002mgYeast 0.02mg(Yeast)
EUR 880

Recombinant Bacillus cereus Putative 3-methyladenine DNA glycosylase (BCQ_0959)
MBS1071677-01mgEColi 0.1mg(E-Coli)
EUR 850

Recombinant Bacillus cereus Putative 3-methyladenine DNA glycosylase (BCQ_0959)
MBS1071677-01mgYeast 0.1mg(Yeast)
EUR 1025

Recombinant Bacillus cereus Putative 3-methyladenine DNA glycosylase (BCA_0925)
MBS1035197-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1125

Recombinant Bacillus cereus Putative 3-methyladenine DNA glycosylase (BCA_0925)
MBS1035197-002mgEColi 0.02mg(E-Coli)
EUR 705

Recombinant Bacillus cereus Putative 3-methyladenine DNA glycosylase (BCA_0925)
MBS1035197-002mgYeast 0.02mg(Yeast)
EUR 880

Recombinant Bacillus cereus Putative 3-methyladenine DNA glycosylase (BCA_0925)
MBS1035197-01mgEColi 0.1mg(E-Coli)
EUR 850

Recombinant Bacillus cereus Putative 3-methyladenine DNA glycosylase (BCA_0925)
MBS1035197-01mgYeast 0.1mg(Yeast)
EUR 1025

Recombinant Staphylococcus epidermidis Putative 3-methyladenine DNA glycosylase (SE_1919)
MBS1404280-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1125

Recombinant Staphylococcus epidermidis Putative 3-methyladenine DNA glycosylase (SE_1919)
MBS1404280-002mgEColi 0.02mg(E-Coli)
EUR 705

Recombinant Staphylococcus epidermidis Putative 3-methyladenine DNA glycosylase (SE_1919)
MBS1404280-002mgYeast 0.02mg(Yeast)
EUR 880

Recombinant Staphylococcus epidermidis Putative 3-methyladenine DNA glycosylase (SE_1919)
MBS1404280-01mgEColi 0.1mg(E-Coli)
EUR 850

Recombinant Staphylococcus epidermidis Putative 3-methyladenine DNA glycosylase (SE_1919)
MBS1404280-01mgYeast 0.1mg(Yeast)
EUR 1025

Recombinant Mycobacterium fortuitum Putative 3-methyladenine DNA glycosylase
MBS1396107-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1115

Recombinant Mycobacterium fortuitum Putative 3-methyladenine DNA glycosylase
MBS1396107-002mgEColi 0.02mg(E-Coli)
EUR 695

Recombinant Mycobacterium fortuitum Putative 3-methyladenine DNA glycosylase
MBS1396107-002mgYeast 0.02mg(Yeast)
EUR 865

Recombinant Mycobacterium fortuitum Putative 3-methyladenine DNA glycosylase
MBS1396107-01mgEColi 0.1mg(E-Coli)
EUR 830

Recombinant Mycobacterium fortuitum Putative 3-methyladenine DNA glycosylase
MBS1396107-01mgYeast 0.1mg(Yeast)
EUR 1015

Recombinant Wolbachia pipientis wMel Putative 3-methyladenine DNA glycosylase (WD_1110)
MBS1357807-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1100

Recombinant Wolbachia pipientis wMel Putative 3-methyladenine DNA glycosylase (WD_1110)
MBS1357807-002mgEColi 0.02mg(E-Coli)
EUR 695

Recombinant Wolbachia pipientis wMel Putative 3-methyladenine DNA glycosylase (WD_1110)
MBS1357807-002mgYeast 0.02mg(Yeast)
EUR 855

Recombinant Wolbachia pipientis wMel Putative 3-methyladenine DNA glycosylase (WD_1110)
MBS1357807-01mgEColi 0.1mg(E-Coli)
EUR 810

Recombinant Wolbachia pipientis wMel Putative 3-methyladenine DNA glycosylase (WD_1110)
MBS1357807-01mgYeast 0.1mg(Yeast)
EUR 1000

Recombinant Bacillus anthracis Putative 3-methyladenine DNA glycosylase (BAA_0975)
MBS1101991-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1125

Recombinant Bacillus anthracis Putative 3-methyladenine DNA glycosylase (BAA_0975)
MBS1101991-002mgEColi 0.02mg(E-Coli)
EUR 705

Recombinant Bacillus anthracis Putative 3-methyladenine DNA glycosylase (BAA_0975)
MBS1101991-002mgYeast 0.02mg(Yeast)
EUR 880

Recombinant Bacillus anthracis Putative 3-methyladenine DNA glycosylase (BAA_0975)
MBS1101991-01mgEColi 0.1mg(E-Coli)
EUR 850

Recombinant Bacillus anthracis Putative 3-methyladenine DNA glycosylase (BAA_0975)
MBS1101991-01mgYeast 0.1mg(Yeast)
EUR 1025

Recombinant Rickettsia bellii Putative 3-methyladenine DNA glycosylase (A1I_05810)
MBS1179793-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1140

Recombinant Rickettsia bellii Putative 3-methyladenine DNA glycosylase (A1I_05810)
MBS1179793-002mgEColi 0.02mg(E-Coli)
EUR 725

Recombinant Rickettsia bellii Putative 3-methyladenine DNA glycosylase (A1I_05810)
MBS1179793-002mgYeast 0.02mg(Yeast)
EUR 890

Recombinant Rickettsia bellii Putative 3-methyladenine DNA glycosylase (A1I_05810)
MBS1179793-01mgEColi 0.1mg(E-Coli)
EUR 870

Recombinant Rickettsia bellii Putative 3-methyladenine DNA glycosylase (A1I_05810)
MBS1179793-01mgYeast 0.1mg(Yeast)
EUR 1045

Recombinant Mycobacterium sp. Putative 3-methyladenine DNA glycosylase (Mkms_3000)
MBS1248796-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1125

Recombinant Mycobacterium sp. Putative 3-methyladenine DNA glycosylase (Mkms_3000)
MBS1248796-002mgEColi 0.02mg(E-Coli)
EUR 705

Recombinant Mycobacterium sp. Putative 3-methyladenine DNA glycosylase (Mkms_3000)
MBS1248796-002mgYeast 0.02mg(Yeast)
EUR 875

Recombinant Mycobacterium sp. Putative 3-methyladenine DNA glycosylase (Mkms_3000)
MBS1248796-01mgEColi 0.1mg(E-Coli)
EUR 845

Recombinant Mycobacterium sp. Putative 3-methyladenine DNA glycosylase (Mkms_3000)
MBS1248796-01mgYeast 0.1mg(Yeast)
EUR 1025

Recombinant Mesorhizobium sp. Putative 3-methyladenine DNA glycosylase (Meso_1432)
MBS1288912-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1100

Recombinant Mesorhizobium sp. Putative 3-methyladenine DNA glycosylase (Meso_1432)
MBS1288912-002mgEColi 0.02mg(E-Coli)
EUR 700

Recombinant Mesorhizobium sp. Putative 3-methyladenine DNA glycosylase (Meso_1432)
MBS1288912-002mgYeast 0.02mg(Yeast)
EUR 855

Recombinant Mesorhizobium sp. Putative 3-methyladenine DNA glycosylase (Meso_1432)
MBS1288912-01mgEColi 0.1mg(E-Coli)
EUR 810

Recombinant Mesorhizobium sp. Putative 3-methyladenine DNA glycosylase (Meso_1432)
MBS1288912-01mgYeast 0.1mg(Yeast)
EUR 1000

Recombinant Rhizobium loti Putative 3-methyladenine DNA glycosylase (mll4583)
MBS1316525-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1125

Recombinant Rhizobium loti Putative 3-methyladenine DNA glycosylase (mll4583)
MBS1316525-002mgEColi 0.02mg(E-Coli)
EUR 710

Recombinant Rhizobium loti Putative 3-methyladenine DNA glycosylase (mll4583)
MBS1316525-002mgYeast 0.02mg(Yeast)
EUR 880

Recombinant Rhizobium loti Putative 3-methyladenine DNA glycosylase (mll4583)
MBS1316525-01mgEColi 0.1mg(E-Coli)
EUR 855

Recombinant Rhizobium loti Putative 3-methyladenine DNA glycosylase (mll4583)
MBS1316525-01mgYeast 0.1mg(Yeast)
EUR 1030

Recombinant Bacillus subtilis Putative 3-methyladenine DNA glycosylase (yxlJ)
MBS1024316-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1115

Recombinant Bacillus subtilis Putative 3-methyladenine DNA glycosylase (yxlJ)
MBS1024316-002mgEColi 0.02mg(E-Coli)
EUR 695

Recombinant Bacillus subtilis Putative 3-methyladenine DNA glycosylase (yxlJ)
MBS1024316-002mgYeast 0.02mg(Yeast)
EUR 870

Recombinant Bacillus subtilis Putative 3-methyladenine DNA glycosylase (yxlJ)
MBS1024316-01mgEColi 0.1mg(E-Coli)
EUR 835

Recombinant Bacillus subtilis Putative 3-methyladenine DNA glycosylase (yxlJ)
MBS1024316-01mgYeast 0.1mg(Yeast)
EUR 1015

Recombinant Rickettsia bellii Putative 3-methyladenine DNA glycosylase (RBE_0390)
MBS1487263-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1140

Recombinant Rickettsia bellii Putative 3-methyladenine DNA glycosylase (RBE_0390)
MBS1487263-002mgEColi 0.02mg(E-Coli)
EUR 725

Recombinant Rickettsia bellii Putative 3-methyladenine DNA glycosylase (RBE_0390)
MBS1487263-002mgYeast 0.02mg(Yeast)
EUR 890

Recombinant Rickettsia bellii Putative 3-methyladenine DNA glycosylase (RBE_0390)
MBS1487263-01mgEColi 0.1mg(E-Coli)
EUR 870

Recombinant Rickettsia bellii Putative 3-methyladenine DNA glycosylase (RBE_0390)
MBS1487263-01mgYeast 0.1mg(Yeast)
EUR 1045

Recombinant Mycobacterium sp. Putative 3-methyladenine DNA glycosylase (Mmcs_2956)
MBS1377424-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1125

Recombinant Mycobacterium sp. Putative 3-methyladenine DNA glycosylase (Mmcs_2956)
MBS1377424-002mgEColi 0.02mg(E-Coli)
EUR 705

Recombinant Mycobacterium sp. Putative 3-methyladenine DNA glycosylase (Mmcs_2956)
MBS1377424-002mgYeast 0.02mg(Yeast)
EUR 875

Recombinant Mycobacterium sp. Putative 3-methyladenine DNA glycosylase (Mmcs_2956)
MBS1377424-01mgEColi 0.1mg(E-Coli)
EUR 845

Recombinant Mycobacterium sp. Putative 3-methyladenine DNA glycosylase (Mmcs_2956)
MBS1377424-01mgYeast 0.1mg(Yeast)
EUR 1025

Recombinant Synechococcus sp. Putative 3-methyladenine DNA glycosylase (sync_0342)
MBS1396184-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1130

Recombinant Synechococcus sp. Putative 3-methyladenine DNA glycosylase (sync_0342)
MBS1396184-002mgEColi 0.02mg(E-Coli)
EUR 715

Recombinant Synechococcus sp. Putative 3-methyladenine DNA glycosylase (sync_0342)
MBS1396184-002mgYeast 0.02mg(Yeast)
EUR 885

Recombinant Synechococcus sp. Putative 3-methyladenine DNA glycosylase (sync_0342)
MBS1396184-01mgEColi 0.1mg(E-Coli)
EUR 860

Recombinant Synechococcus sp. Putative 3-methyladenine DNA glycosylase (sync_0342)
MBS1396184-01mgYeast 0.1mg(Yeast)
EUR 1035

Recombinant Nocardia farcinica Putative 3-methyladenine DNA glycosylase (NFA_19900)
MBS1383251-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1130

Recombinant Nocardia farcinica Putative 3-methyladenine DNA glycosylase (NFA_19900)
MBS1383251-002mgEColi 0.02mg(E-Coli)
EUR 715

Recombinant Nocardia farcinica Putative 3-methyladenine DNA glycosylase (NFA_19900)
MBS1383251-002mgYeast 0.02mg(Yeast)
EUR 885

Recombinant Nocardia farcinica Putative 3-methyladenine DNA glycosylase (NFA_19900)
MBS1383251-01mgEColi 0.1mg(E-Coli)
EUR 860

Recombinant Nocardia farcinica Putative 3-methyladenine DNA glycosylase (NFA_19900)
MBS1383251-01mgYeast 0.1mg(Yeast)
EUR 1035

Recombinant Clostridium tetani Putative 3-methyladenine DNA glycosylase (CTC_01030)
MBS1439143-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1125

Recombinant Clostridium tetani Putative 3-methyladenine DNA glycosylase (CTC_01030)
MBS1439143-002mgEColi 0.02mg(E-Coli)
EUR 705

Recombinant Clostridium tetani Putative 3-methyladenine DNA glycosylase (CTC_01030)
MBS1439143-002mgYeast 0.02mg(Yeast)
EUR 875

Recombinant Clostridium tetani Putative 3-methyladenine DNA glycosylase (CTC_01030)
MBS1439143-01mgEColi 0.1mg(E-Coli)
EUR 845

Recombinant Clostridium tetani Putative 3-methyladenine DNA glycosylase (CTC_01030)
MBS1439143-01mgYeast 0.1mg(Yeast)
EUR 1025

Furthermore, each rrs gene and different phylogenetic analyses confirmed that distinctive clades of the Lachnospiraceae, Bacteroidales, and Gammaproteobacteria are predominant within the Tammar foregut microbiome. Nucleotide composition-based sequence binning facilitated the assemblage of greater than two megabase pairs of genomic sequence for one of the novel Lachnospiraceae clades (WG-2). These analyses present that WG-2 possesses quite a few glycoside hydrolases focusing on noncellulosic polysaccharides.

 

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